Showing posts with label lose fat. Show all posts
Showing posts with label lose fat. Show all posts

Monday, December 30, 2013

Forskolin: Friend or Foe? Stories and Studies About Fat Loss, Lean Gains, Topical Cellulite Treatment, Testosterone, Cancer, Hepatotoxicity, Drug Interactions & More

There is a single human study that would suggest that forskolin would make you get closer to this classic physique w/out tons of salad (who said that's necessary anyway?).
Since Maxim asked in one of his more recent comments about the usefulness and/or downsides of forskolin, I dediced to dedicate this Sunday (finally again?) to answering a user question and am going to briefly sum up some older and the few novel findings on forskolin I am aware of.

For those of you who find that boring: Don't blame Maxim alone, another reason for this decision was that I have seen discussions on forskolin resurface elsewhere on the Internet. By the way, I write re-surfaced, because forskolin has once been hailed as a testbooster and fat loss adjuvant, but as the prices increased and people came out with faked or low-quality products that did not yield results, the market collapsed.

What is forskolin and where does it originate from?

As usually there is more than a single answer to this question. The most straight forward general ones are probably (a) it is a white to white with yellow cast powder, or (b) a labdane diterpenoid with antihypertensive, positive inotropic, platelet aggregation inhibitory and adenylate cyclase activating properties. Moreover, forskolin is able to activate the adenylate cyclase and thus increase the intracellular cyclic AMP levels in most tissues and cells. And hat  it's called forskolin, because it is derived from the Indiant plan Coleus forskohlii is probably something 99% of you knew already.

The reason I suppose that Maxim got interested in it, is that it is commonly used in cell studies to raise the levels of cyclic AMP (cAMP; cf. Alasbahi. 2013) and did a pretty impressive job in the recently discussed PGC-1a study. On the other hand, it did also increase the expression of the aromatase enzyme in the Yang study mentioned in the "Natural Sildenafil & Testosterone Alternative" post on which Maxim replied with the initially mentioned comment.

"Wait, wasn't it supposed to be a testbooster and now it also inhibits myostatin and increases estrogen? What does this stuff not do?" - Well, forskolin is, above all, a cAMP modulator

Forskolins chemical structure. Sometimes it's also referred to as Colforsin; 7-beta-acetoxy-8, 13-epoxy-1-alpha, 6-beta, 9-alpha-trihydroxylabd-14-en-11-one; or Coleonol (img. from Sigma-Aldrich's product database)
I know that sounds confusing, but in essence forskolin does nothing but increasing cAMP levels in almost all types of cells. cAMP a breakdown product of ATP (=> cAMP => AMP) in turn is one of those molecules which exert most their effects as intracellular signal transducer. In that, it is involved in the activation of protein kinases and regulates the effects of adrenaline and glucagon. It also modulates the calcium channels and contributes to growth hormone release; unfortunately, cAMP has also been implicated in the proliferation of not very beneficial cell growth aka cancer. The same ion-flux mediation has recently been implicated in the etiology of ADHD, as well (Arnsten. 2013).

Still, it's not all about c-AMP. Probably cAMP unrelated downsides of coleus forkohlii are for example:
  • forskolin induces hepatic CYP2C enzymes and coleus forskohlii extract and thus attenuates the anticoagulant action of warfarin. (Yokotan. 2013) 
  • even more than isolated forskolin, coleus forskohlii  messes with the hepatic enzyme cascade (P450) and has even been shown to be hepatoxic in a study published in the July issue of the Journal of Toxicology (Virgona. 2013)
On the other hand there are a handful of benefits, e.g.
  • Figure 1: Effects of 12 weeks on 2x250mg (10%) forskolin on testosterone (free and total) and lean & fat mass (Godard. 2005)
    In a 2005 study (Godard. 2005), which caused quite a stir in the health and fitness community back then, Godard et al. observed profound beneficial effects of testosterone and body composition (cf. figure 1) after the ingestion of 2x250mg of a 10% standardized forskolin (Forslean).

    Now, the unfortunate truth is that the15 subjects (average age, BMI, and body fat percent were 24.4 +/- 5.9 years, 32.5 +/- 4.1 kg/m2 , and 35.2 +/- 8.3%) who had been randomized to the active arm of the study, and the 15 participants in the placebo arm (28.7 +/- 8.6 years, 32.6 +/- 3.8 kg/m2 , and 35.0 +/- 7.3%) were non-active sedentary overweight/obese (BMI 26 kg/m2 or more) individuals. Add the funding by Sabinsa (Forslean producer) to the equation and decide for yourself how relevant you think the results are going to be for you...
  • In several in-vitro studies, forskolin has been used as a positive control to compare the effects of other compounds on the testosterone release in leydig cells. Lin et al. for example used it in 2001 as a comparison for lactate and found a ~3x increase in testosterone release in incubated leydig cells (Lin. 2001). A similar study by Yu et al. showed that the addition of green tea catechins lead to an additional stimulation of forskolin induced testosterone production in cell cultures (Yu. 2010).
  • Figure 2: Results of 12-week intervention w/ forskolin containing topical cream (Roure. 2011)
    As part of a topical cosmetic slimming product combining tetrahydroxypropyl ethylenediamine, caffeine, carnitine, retinol and, obviously, forskolin it has shown some promise as a topical anti-cellulite and toning agent (Roure. 2011). The clinical study was however financed by Johnson & Johnson and I am not sure how much of the effects were actually brought about by forskolin (the placebo was a basic gel with the same texture containing mainly water, gelifying and preservative systems). So take the data in figure 2 with a grain of salt, ladies - I bet 12 weeks on this product are not going to be exactly inexpensive.
    • The administration of forskolin in conjunction with rutin (the glycoside between the flavonol quercetin and the disaccharide rutinose), vitamin B1 & B2 in a 2010 study by Pescosolido et. al. lead to a significant reduction in intra-ocular pressure in 15 glaucoma patients after 40 days (Pescosolido. 2010). Similar results were observed in a 2013 study for forskolin and rutin alone (Vetrugno. 2013)
    • An in-vitro study by Cristobal et al. provides first evidence for the ability of forskolin to restore PPA2 in acute myeloid leukemia. That would make it a potential candidate for the treatment of this type of cancer, but to my knowledge there is as of yet not even a rodent study that would support these in-vitro results. Moreover, previous studies have suggested that Forskolin may even favor the proliferation of other types of leukemia (Kobayashi. 1994)
        Time to weigh the "established" benefits and downsides

        Figure 3: Effect of different doses of forskolin with and w/out epinephrine on FFA release from rat adipocytes - watch out this is from yet another in-vitro study with rodent cells (Litosch. 1982)
         In view of the fact that the aforementioned study by Godard is the only human study is only backed up by in-vitro data from rodent studies (Litosch. 1982, cf. figure 3), the fat loss benefits are as  Jeukendrup et al. point out in their 2011 review of purported fat burners...
        "[...] promising, there is [yet] only one study at the present time and more work is required before forskolin can be recommended as a fat metabolism-enhancing substance." (Jeukendrup. 2011)
        If you add to this the host of wanted and unwanted, known and unknown side effects that occur in response to the coleus foskohlii induced cytochrome P450 modulation (e.g. the mice in the aforementioned study by Virgona lost some visceral fat, but the costs were increased fat deposition in the liver and elevated transaminase levels).

        With the questionable "fat loss" benefits (remember stress is also a powerful lypolitic and the problem is not to get the fat out of the cell, but rather to burn it), and the almost non-existant human data on the purported testosterone boosting effects, this should be reason enough not to buy more than one bottle for a test-run. After which I highly suggest to do some lab work to see if whatever good or bad you believe you are feeling is an actual boost in T (check T-levels) or hepatic side effects (check ALT, AST & ALP).

        Note (update in response to comments): As far as the hepatoxicity is concerned the suggested dosage of 2x 250mg coleus forskholii most supplements come with may be higher than the medium dose in the study by Virgona, but is still probably "liver save" if you double dose on that, you are however landing in the no-man's land (=not tested for) gray zone between the medium dosage and the "danger zone" of  ~49mg/kg per day (human dose equivalent) that was tested in the study. Don't freak out, if you did that in the past, the levels return to normal afterwards and temporarily elevated ALT + AST or ALP levels do not necessarily mean that your liver is whacked forever ;-)

        References:
        • Alasbahi RH, Melzig MF. Forskolin and derivatives as tools for studying the role of cAMP. Pharmazie. 2013 Jan;67(1):5-13.
        • Arnsten AF, Jin LE. Guanfacine for the treatment of cognitive disorders: a century of discoveries at Yale. Yale J Biol Med. 2013 Mar;85(1):45-58. Epub 2013 Mar 29.
        • Godard MP, Johnson BA, Richmond SR. Body composition and hormonal adaptations associated with forskolin consumption in overweight and obese men. Obes Res. 2005 Aug;13(8):1335-43. 
        • Jeukendrup AE, Randell R. Fat burners: nutrition supplements that increase fat metabolism. Obes Rev. 2011 Oct;12(10):841-51. 
        • Kobayashi K, Nishikawa M, Omay SB, Toyoda H, Deguchi K, Shirakawa S. Forskolin potentiates G-CSF-induced proliferation of a murine myeloblastic leukemia cell line. Leuk Res. 1994 Feb;18(2):111-7.
        • Lin H, Wang SW, Wang RY, Wang PS. Stimulatory effect of lactate on testosterone production by rat Leydig cells. J Cell Biochem. 2001 Jun 26-Jul 25;83(1):147-54.
        • Pescosolido N, Librando A. Oral administration of an association of forskolin, rutin and vitamins B1 and B2 potentiates the hypotonising effects of pharmacological treatments in POAG patients. Clin Ter. 2010;161(3):e81-5. 
        • Roure R, Oddos T, Rossi A, Vial F, Bertin C. Evaluation of the efficacy of a topical cosmetic slimming product combining tetrahydroxypropyl ethylenediamine, caffeine, carnitine, forskolin and retinol, In vitro, ex vivo and in vivo studies. Int J Cosmet Sci. 2011 Dec;33(6):519-26.
        • Vetrugno M, Uva MG, Russo V, Iester M, Ciancaglini M, Brusini P, Centofanti M, Rossetti LM. Oral administration of forskolin and rutin contributes to intraocular pressure control in primary open angle glaucoma patients under maximum tolerated medical therapy. J Ocul Pharmacol Ther. 2013 Oct;28(5):536-41.
        • Virgona N, Taki Y, Yamada S, Umegaki K. Dietary Coleus forskohlii extract generates dose-related hepatotoxicity in mice. J Appl Toxicol. 2013 Jun 22.
        • Yokotani K, Chiba T, Sato Y, Taki Y, Yamada S, Shinozuka K, Murata M, Umegaki K. Hepatic cytochrome P450 mediates interaction between warfarin and Coleus forskohlii extract in vivo and in vitro. J Pharm Pharmacol. 2013 Dec;64(12):1793-801.
        • Yu PL, Pu HF, Chen SY, Wang SW, Wang PS. Effects of catechin, epicatechin and epigallocatechin gallate on testosterone production in rat leydig cells. J Cell Biochem. 2010 May 15;110(2):333-42.

        Wednesday, December 25, 2013

        Beyond Celiac: Study Sheds New Light on Obesogenic Effects of Gluten - Are PPARs & Bacteria Both Involved?

        Cornflakes peanut butter cookies - guaranteed not gluten free ;-)
        With Christmas Eve being over, and grandma's cookies, Christmas stollen, and all sorts of other stuff from the bakery in front of you (literally), Christmas Day may actually prove to be a way more "dangerous" than Christmas Eve - not just because of the total amount of calories, but also because of the low satiety effect of these sweet treats.

        A recent paper by scientists from the Universidade Federal de Minas Gerais in Belo Horizonte in Brazil does now point to another reason you better give those bakery products a wide berth - not just, but especially with the energy overshoot on Christmas day: Gluten!

        Study confirms for the first time what scientists and laymen alike have been speculating about

        In what the scientists claim is the first well-controlled study of the effects of gluten intake on metabolic health in a non-celiac, but Western-style diet scenario, Fabíola Lacerda Pires Soares and her colleagues put two groups of C57BL/6 mice on identical, iso-caloric high fat (hypercaloric) diets that differed only in terms of the amount of gluten that was added to the chow (0% gluten vs. 4.5% gluten).

        Interestingly, the gluten diet did not influence any of the usual suspects, like food intake, total fat-free mass, fecal lipids excretion, blood lipid profile, blood total protein and ectopic (liver and muscle) lipid concentration (if you look closely you will realize that the gluten-free group actually had higher TRIGs, although the difference did not reach statistical significance).
        Figure 1: Usual suspects and closer look at the effects 8 weeks gluten supplemented vs. gluten-free diets had on serum markers of metabolic syndrome and visceral fat parameters (Soares. 2013)
        The data in figure 1 (right) does yet also show that the gluten content of the diet did nevertheless have a significant impact on the total body mass, visceral fat mass, lipid content and most importantly the adipocyte size.
        Figure 2: Absolute adipokine levels (left) and fasting glucose and insulin levels, as well as Homa-IR (Soares. 2013)
        Add to that the blunted expression of the anti-inflammatory and anti-diabetic fat hormone adiponectin and the increased the >5x higher expression of leptin (figure 2). And mix that with the reduced expression of PPAR-alpha and gamma of which Soares et al. argue that they may well be the key factor in the detrimental modulatory effect the addition of gluten had on the visceral fat structure and the lowered expression of the fat liberating enzymes LPL and and HSL, as well as reduced levels of the fat burning proteins ACC and CPT-1 (figure 3).
        Figure 3: PPAR-alpha, -gamma, LPL, HSL, ACC and CPT-1 expression compared to rodents on regular chow (left); crown like structures in stained slices from visceral fat, inflammatory markers TNF-alpha and IL-6 (Soares. 2013)
        So, even if the initially mentioned blood markers (aka the usual suspects) would suggest that both the gluten-consuming and gluten-free rodents were similarly bad off, the profound difference in inflammatory markers within the adipose tissue and the presence of comparatively many necrotic and inflammatory adipocytes in the crown like structures stand in line with increases in HOMA-IR, fasting glucose and insulin and an already compromised glucose clearance which are well-known harbingers of the metabolic syndrome.

        These observations do not simply shed a whole new light on a hitherto largely ignored contributer to the etiology of the metabolic syndrome, they do also show that one of the reasons it has not been identified before is an over-reliance on BMI, total fat mass and serum lipids in the early stages of diabesity.

        Reardless of whether the gut microbiome is part of the mechanism by which gluten predisposes the development of metabolic syndrome. Eating more inulin- and beta-glucan rich foods like Jerusalem artichokes, agave, bananas, onion, steel cut oats, wild yams, yacon, etc. certainly won't hurt your efforts to get lean, stay lean and leave the role of the obese diabetic to the other (read more)
        Bottom line: The study at hand provides a good reason to limit your intake of "healthy whole grains" and other gluten containing foods, regardless of whether you suffer from celiac or not. Whether the established detrimental effects of gluten on the integrity of the intestinal wall and the increased leakage of bacterially produced endotoxins from the highly unfavorably changes in the gut microbiome in response to the high fat diets (Hildebrandt. 2009) are part of, or even the primary cause of these observations still has to be elucidated. The same goes for strategies to counter the translocation of the endotoxins across the gut lining (cf. "Shedding some light on the leaky gut") and the dose response relationship between the total amount of gluten in your diet and its effects on your metabolism. With 7% of pure gluten, it goes without saying that you would basically have to live of wheat in order to get to anywhere similar amounts of gluten in the diet... that said: Is it possible that the effects occur only in the presence of the high fat diet? After all, this alone has been shown to favor a pro-inflammatory gut microbiome.

        You see there are enough questions to be answered in 2013 and the SuppVersity is going to be the place you will read the respective answers first ;-)

        References:
        • Hildebrandt MA, Hoffmann C, Sherrill-Mix SA, Keilbaugh SA, Hamady M, Chen YY, Knight R, Ahima RS, Bushman F, Wu GD. High-fat diet determines the composition of the murine gut microbiome independently of obesity. Gastroenterology. 2009 Nov;137(5):1716-24.e1-2.
        • Soares FL, de Oliveira Matoso R, Teixeira LG, Menezes Z, Pereira SS, Alves AC, Batista NV, de Faria AM, Cara DC, Ferreira AV, Alvarez-Leite JI. Gluten-free diet reduces adiposity, inflammation and insulin resistance associated with the induction of PPAR-alpha and PPAR-gamma expression. J Nutr Biochem. 2013 Dec 17.

        Tuesday, December 24, 2013

        "Mini-Fast With Exercise" a la Intermittent Fasting Can Help Minimize the "Damage" During the Holiday Season

        I wonder if it is coincidence that the modern Santa is an obese advertisement character invented by some marketing genius working for Coca Cola?
        Those of you who have been reading the intermittent thoughts on intermittent fasting (click here and start with the links at the bottom, if you haven't) will remember that the actual number of studies on intermittent fasting, as it is interpreted by most people on the Internet is actually quite scarce. Aside from the Ramadan studies, you mostly see very long fasting periods or other oddities that decrease the significance of the studies. That said, I did hit onto an older study that has been published in Medical Hypothesis in 2009, already. A study with a daily 12-14h "mini-fast" + low intensity aerobic exercise 5x a week and a study the results of which point to the usefulness and practical value of skipping a meal - especially in a time where you give in to nutritional temptations way too easily, anyhow - the holiday season!

        Fast, walk, eat and stay lean during the holiday season and afterwards

        Especially for those of you who are having some weight issues anyway, the profound body fat loss, the subjects in the Bahadori study which was based on a 7 step program comprising
          * Better than monitoring total fat and GI would be to monitor your N-6 intake, emphasize mono-saturated fatty acids and eyeball the total glycemic load (the latter means potatoes are ok, but cookies are a "better stick to one" item ;-)
        • a mini-fast with exercise (12–14 h) fast during every 24 h + prolonged, moderate-intensity aerobic exercise (e.g. 40-45 minutes of brisk walking) Meals are not
        • no caloric restrictions, but a focus on low fat, low GI meals*
        • an increased consumption in calorie-free beverages during the fast
        I know that sounds stupid and 100% mainstream, but if you take a look at the data in figure 1 you will notice that it worked like a charm... well, at least for the 10 overweight men and 17 women who followed this protocol for 12 months.
        Figure 1: Body weight and composition, waist and insulin levels from the beginning to the end of the 12 week non-calorically restricted 10-14 hour fast with light aerobic exercise; the asterisk (*) behind body fat (%) and insulin signifies that these values are plotted on the 2nd-ary axes (Bahadori. 2009).
        Personally, I believe that one, if not the reason that this protocol worked so well were it's feasibility and flexibility, as well as being allowed to eat to satiety, when the subjects were not fasting. The 27 subjects who participated in the study were also free to chose whether they wanted to skip breakfast or dinner and switch from one to the other strategy on a daily basis to adapt their diet to their working hours and other obligations:
        Coffee and teas like green, black oolong and pu-erh are your friends not just during the mini-fasts (read more).
        "If participants choose to exercise in the morning, they skip breakfast and do not eat until noon. If they choose to exercise at noon, they skip lunch. If they choose to exercise in the evening, they eat an early light dinner, and then wait at least two hours to exercise (so that fasting insulin levels are restored); no food is consumed between the exercise and bedtime. (A variant of this latter strategy is to eat no dinner at all; several volunteers in the study described below adopted this approach on their own initiative.) To optimize the flexibility of this regimen, participants are allowed to switch the time of their exercise session from day to day." (Bahadori. 2009)
        For your personal Christmas fast this could mean you skip breakfast tomorrow (which should not be a problem with the large Christmas dinner in your tummy), go out for a long brisk walk, have a light dinner, like a mixed salad with some cheese and chicken breast, and thus prepare yourself for the next family feast on Christmas day. If you are brunching on the day after Christmas, not a problem, you just sleep out, go for light 30min jog, shower and head over to the brunch. Eat as much as you like, and either skip dinner later or breakfast the day after.

        If you are not into coffee and tea, ginger ale is another fasting friendly weight loss drink (read more).
        Bottom line: To keep the damage at bay this the "mini-feast + exercise" protocol is probably feasible for anyone. It's easy, it will allow you to take part in all the Christmas festivities and won't have you sit there like an orthorexic leopard who cannot change his spots .

        Whether this is a strategy worth following for longer depends on where you are at and where you want to go. If you want to look like a fitness model, it's unlikely to be sufficient to reach your goal. You will, for example, have to tweak that regimen to (a) incorporate strength training or add it in later in the day, (b) think about using a couple of supplements, such as BCAAs, caffeine and green tea during the fast and (c) eyeball the quality of the foods you eat with convenience, fast and junk-food being the exceptions to the rule of eating self-prepared whole food meals.

        There are however certain downsides to protocols like this, a potential increase in adipogenesis (22% vs. 12% fat gain) and decreased lean gains (50% vs. 72%), as they were observed in a rodent study by Verbaeyes et al. (see "Eating by The Clock Could Make You Fat") with unrestricted caloric intake within the 6h feeding window, would be an example and evidence that whenever you are effectively trying to gain weight you are probably better off spreading your food intake more evenly across the day. Alternatively, you can enlarge the feeding window to 10h to maximize lean gains without the negative side effects of force-feeding yourself with 2x1,500kcal meals only twice a day or grazing continuously for 6h to meet your caloric demands.

        But hey, Christmas is almost there, and there is still enough time to think about dieting and bulking after the festivities. On that note, I wish all of you a happy Christmas, a nice time with your families, friends! Regardless if you fast or not ;-)

        References:
        • Bahadori B, McCarty MF, Barroso-Aranda J, Gustin JC, Contreras F. A "mini-fast with exercise" protocol for fat loss. Med Hypotheses. 2009 Oct;73(4):619-22.

        Monday, December 23, 2013

        Making HIIT a Hit Part II/II: Training Optimization? Where Do I Start? Theoretical Considerations & 10 Rules of Thumb

        The stop watch is your friend, when it comes to HIIT optimization.
        After the examples selected examples in the previous installment the intention of this 2nd part of the serious is to find some more general rules of HIIT program optimization with a special focus on exercise and performance on the one hand and fat loss and improvements in body composition on the other hand.

        Now, if you want to optimize something, you often have one or a set of parameters you can plug into a system of linear equations to find those parameters which generate the maximal output.

        Unfortunately, we are not only lacking linear, but any form of exact equations, but are also at a loss how programming is actually done. In that, Laursen's & Jenkins' definition of optimization as identifying
        "[...] the optimal exercise intensity, exercise duration and number of interval bouts, in addition to the type (active vs passive) and duration of the recovery between exercise bouts."
        May be helpful, but leaves us with the problem that
        "[t]hese variables require manipulation according to the periodisation phase of annual training programmes, training status and the individual response that an athlete has to a training stimulus." (Laursen. 2002)
        In essence, although more than 10 years have passed since the publication of this paper and the number of pertinent studies has increased, we are still (and will probably always be) at a loss when it comes to identify a single best HIIT protocol that will yield optimal results for everyone.

        How do we define intensity?

        In fact, even the way by which the exercise intensity is best prescribed, has as of now not been standardized. The most popular way probably is by VO2Max levels, but there are alternatives which could in fact be quite useful, e.g.:
        • Your exercise selection should mirror the demands of the sport you are training for. Anyone competing in one of the classic team sports, will probably benefit most from sprints (either outside or on the treadmill). A cyclist will use the bicycle and a rower the rowing machine. Don't use equipment where you have to tweak the resistance not the speed (e.g. many of the old crosstrainers) to increase the intensity. HIIT is supposed to be fast and thus hard, not hard and thus slow!
          anaerobic threshold - intensity at which the exercise become predominantly anaerobic
        • T_vent, T_lac - time to ventilatory threshold, time to lactate threshold
        • OBLA - The onset of blood lactate accumulation  
        • critical power - intensity that can be maintained for 30-60min
        • V_max - min.speed required to reach the ventilatory threshold in an incremental exercise test
        • T_max - time an athlete can run at Vmax
        Now, let's be honest. How many of you know their VO2max? Or the time and speed at which you get there? If we simply assume that V_max is about 90% of what you would call "all-out" it does yet appear that the T_max value was one of the better ways to prescribe the training intensity during high intensity interval training (cf. Billat. 1998). Since there is no clearcut relation between VO2Max, V_max and T_max (25% inter-individual variation of T_max in athletes with identical VO2max), anyways, this appears to be the most reasonable and practical approach - also because measuring 60% of T_max (assuming we approximate it as the time at 90% of all out) requires nothing but a stop watch and can be repeated on a monthly basis to adapt the training intensity.

        Training for increased endurance performance and conditioning

        According to studies by Billat et al. and Smith et al.  (Billat. 1999, Smith. 2003) sprints lasting 50% (1:1 work to rest ratio) and 60% (1:2 work to rest ratio) of the T_max are not just sufficient, but according to Smith et al. even superior to intervals with a length of 70% of the T_max and 1:2 work to rest ratios. Although both studies were conducted in highly trained individuals, it is likely that the T_max, which is propably way lower for recreationally active, let alone sedentary individuals, provides an appropriate gauge for "optimal" training intensities in this subgroup as well. After all, this would mean that trained athletes require longer interval durations to further augment their performance than Joe Average.
        Table 1: Summary of findings in high-intensity interval-training (HIT) studies in highly trained cyclists. 3-HCoA = 3-hydroxyacyl coenzyme A dehydrogenase activity; CHOOX = carbohydrate oxidation rates; CS = citrate synthase activity; HK = hexokinase activity; PFK = phosphofructokinase activity; Ppeak = peak aerobic power output; TF100 = time to fatigue at 100%; TF150 = time to fatigue at 150% of Ppeak; TT40 = 40km time-trial performance;
        β = buffering capacity; ↓ = decrease; ↑ = increase; ↔ = no change. (adapted from Laursen. 2002)
        And in fact, the currently available research appears to confirm this, while evidence from HIIT studies in trained cyclists suggests that the same HIIT protocols in the 5s-60s range at 80-90% of all-out performance are probably insufficient to induce further performance increases in already trained subjects (see table 1, rows marked red), unless the intensity is upped to supramaximal levels (see table 1, last row).

        Optimal interval length, interval to rest ratios and recovery times

        A T_max based exercise prescription with interval lengths of 50-60% (not more) of T_max, would probably mirror these increases in "optimal" interval length from non-trained over recreationally trained to elite athletes and could therefore provide a more accurate way of planning your HIIT sessions than picking a fixed work and rest duration.

        Figure 1: Effects of different rest periods between 40m sprints on sprint performance (Balsom. 1992)
        Let's assume you could go for just 2 min at a 80% pace of what you perceive as all-out. You would end up at a 1min interval duration, while your body, a highly trained sprinter can maintain the very same intensity for 5 minutes will have to go for intervals of 2.5min. For both an interval to rest ratio of 1:2 seems to be a happy medium, at very short submaximal intensities (in this case 80%). As the data in figure 1 goes to show, the rest requirements can be profoundly increased for all-out 40m sprints with 60s rest after a 5.6-5.7s sprint equaling a sprint interval to rest ratio of 1:11 in "seven moderately to well-trained highly motivated male physical education students" (Balsom. 1992).

        The same 1:2 rule that applied to the T_max 50-60% workouts will thus maybe fail you, when you are going faster / more intense. In this context you may also argue that rest times below 60s - just as it is the case with strength training - must be regarded as an intensity technique that's actually supposed to increase the overload on the musculature by cutting rest in-between sets.

        Moreover, the increased lactate during active recovery phases has repeatedly been shown to be advantageous in terms of both lower lactate accumulation and an increased likelihood of actually achieving VO2max and thus eliciting a strong enough training stimulus to trigger an adaptive response (see review in Billat. 2001; 22-23).

        Can you combine intervals with a strength training routine?

        Now, I would assume that most of you are no endurance athletes. So the aforementioned studies, which either were HIIT stand-alone studies or - in the case of the trained athletes - studies in which the participants replaced 1-2 of their regular aerobic exercise training with HIIT training do not answer the still highly debated question whether you can or cannot do both HIIT and strength training. I have already pointed out in previous posts that doing so on separate days doesn't seem to be a problem. However, if you "HIIT it" on the day you would usually do say chest and back. You would obviously have (a) the time and (b) the regenerative capacity to add another workout day to your week if you did not want to switch from a 3- to a 2-day split.

        Baking soda could help managing the additional load (read more)
        Against that background, I was not really surprised that DoesObamaEvenLift mentioned a pertinent paper by researchers from the University of Sao Paulo in his (or her?) comment beneath the first installment of this series. In this particular study (de Souza. 2013), the researchers wanted elucidate if the combination of HIIT + hypertrophy oriented strength training would hamper the benefits of one or another and whether the molecular adaptations would explain the differential effects on conditioning, strength and body composition of 43 active male physical education students who did not engage in any regular strength and aerobic power/capacity training within the last 6 months.

        The good news for all muscle heads: Despite the differences in molecular adaptations between training regimens, 8 weeks of concomitant training (CT), i.e. the combination of interval training and lifting on one day did not blunt muscle strength and hypertrophy increments when compared with strength training (ST; 8-12RM for a total of leg-press 45 °, knee extension and knee flexion with 90-120s rest between sets) alone. The bad news is however that this protocol has little to do with the workout regimen of the average gymrat, who trains on 3x or more days per week and doesn't do "leg days" (without squats) on each of those days.
        Figure 2: Illustration of the way the scientists tweaked the different parameters of the HIIT protocol to make it gradually more demanding from week 1-8 (left) p-AMPK and p-AKT response to different types of exercise (C: sedentary control; IT: interval training; ST: strength training; CT: concomitant training; based on de Souza. 2013)
        So, while it cannot be said whether muscle or strength gains would be hampered in the long run, the way the scientists tweaked the interval part of the workout from week 1 to week 8 by slowly increasing the running speed 80-100% of the speed at which they had been running in the previous test to achieve VO2max, while increasing the rest between sets and accommodating for the additional strain of going at 95%-100% by cutting back the volume (see figure 2 left) stands in line with what has been said about the complex relation of these parameters in the previous paragraphs.

        Don't forget the progression if you want to make progress

        If your goal is to actually increase your conditioning and VO2max a carefully planned progression is an absolute must. To to so it would appear reasonable to re-test the initially discussed T_max after 6-8 weeks in the course of which you would progress from 80% to 95-100% in a way similar to what the participants in the study did. Based on the change in T_max you would then increase the length of the intervals appropriately while keeping the rest times similar. If your T_max for example had gone up by ~20% during the last cycle, you  would start your 2nd cycle with 72s intervals at 80% with the same progression you have used before.

        HIIT for metabolic benefits and improved body composition

        It's actually quite funny that up until very recently, HIIT training has been completely overlooked by most researchers who are looking for optimal exercise modalities to ameliorate. Within the last 2-5 years or so, the number of studies that suggest that high intensity interval training can do that at least as well, if not better than regular steady state aerobics is yet increasing. Here are a couple of examples, you may or may not remember from the SuppVersity news:
        • Previous studies have shown that a very brief interval training, even when it's performed on an empty stomach, like first thing in the morning or on an intermittent fast is not catabolic, but will in fact increase protein synthesis by 43% in men and  122% in women (read more).
          Some HIIT For Life & Less LISS For More! How to Burn 27,300 Kcal Extra W/out Losing a Single Extra Pound of Fat! (read more)
        • The Iranian HIIT Solution: Three 200m Sprint Sessions per Week Double Insulin Sensitivity & Normalize Leptin Levels (read more)
        • Reduced Exertion High Intensity Training - A Minimalist 2x20s HIIT Protocol For The Male Convenience Generation. (read more)
        • HIIT is the Hit! Interval, not Steady State Aerobics is the Way to Go - Even for Patients with Myocardial Infarctions! (read more)
        • HIIT-ing Health: Positive Effects of High Intensity Training on Symptoms of Metabolic Syndrome. (read more)
        When you go through these studies you will realize that it's impossible to identify the single-best HIIT workout for fat loss, what appears to be clear though, is that fat loss workouts do not require the same long intervals athletes with an already high VO2max need to promote their conditioning.

        While the longer intervals may in the end burn more energy, the previously mentioned increase in lactate suggests that the additional energy expenditure in the course of the workout (a) does not come from your fat stores and (b) could take its toll, when you are dieting. Against that background, an "optimal" HIIT fat loss routine will probably be characterized by shorter, highly intense sprints and longer active recovery between the sets, with the latter giving your ramped up metabolism time to tap into the fat reserves, get rid of the lactate and ready for the next HIIT stimulus.

        Combining HIIT and aerobic training to augment fat loss

        Are You Still Burning Calories or Already Losing Fat? Study Shows: 5x15 Min HIIT Reduce Body Fat & Improve Fitness Twice as Effectively as 5x40min of Classic Cardio (read more)
        A similar rationale is actually behind the idea of combining HIIT and aerobic exercises in a single fat loss workout that consists of an initial HIIT part of 3-5 intervals at 90-95% of what you could be sprinting for 30-45s interspersed by 2-3 minutes of active recovery followed by 15-20min of light intensity steady state of an intensity that would equal walking or very light jogging on a treadmill. With a total workout duration of ~30-45min this should yet be done on a day of it's own.

        A viable alternative for everyone who doesn't want to give up on their current strength training routine and doesn't have room for another training day would be to alternate between the shorter varieties of the HIIT part and the LISS part of the aforementioned workout as an adjunct to a short, but intense resistance training regimen (30-40min total with no more than 18-20 sets).

        How to progress when you are dieting?

        As obligatory as a progression in intensity and duration of the intervals may be for someone who's trying to increase his or her conditioning and VO2max, it is nothing I would suggest on a diet. Unless you are trying to progress from overweight or obese to normal and are in real bad shape, it is not exactly likely that your performance will be increasing to an extend that will require adaptations.

        That said, it is not necessary and in most cases counterproductive to increase the workload, when what you would actually have to do to drop body fat is to reduce your energy intake. Have patience and simply stop dieting, when your progress stalls for 2 weeks (sometimes even an additional refeed can reignite the weight loss).

        Can HIIT help me make lean(er) gains?

        8x Increase in "Mitochondria Building" Protein PGC1-Alpha in Glycogen Depleted Elite(!) Cyclists: Training Revolution or Recipe for Disaster?  (read more)
        Personally, I have had greater success with very light steady state cardio like walking on an inclined treadmill either in the morning or after a strength workout, but if you take a look at the aforementioned SuppVersity post about increase protein synthesis (see image with sprinting guy and girl next to the list of examples), or take both the results of the Psilander study that was on the news in October 2013 and the latest revelations about PGC-1 A4 and how it may benefit from pre-workout glycogen depletion, a short but intense HIIT training right before your workout may not only help you to stay lean, but could actually improve your progress - assuming you don't end up seriously overtrained and are willing to eat enough.

        Are there any supplements that can help?

        Aside from the regular fat loss adjuvants, a combination of creatine, caffeine and amino acids, as it was used in a 2010 study by Smith et al. would be a basic stack to increase your HIIT and overall workout performance and thus augment body fat loss. If you use BCAAs this would also make a good addition to any fasted HIIT regimen in the morning for those of you who follow an intermittent fasting protocol.
        Figure 3: Lactate, pH, ratio of hydrogen carbonate ions to NaHCO(3) and base excess in blood after, as well as number of total reps performed during a leg workout (data adapted from Carr. 2013 - discussed on 09/05/12)
        Also, the addition of baking soda, may be worth trying, because the increased alkalinity with concomitantly increased workout intensities could provide both metabolic (=lower propensity to gain fat) and performance (=increased growth stimulus) advantages.

        Too many stimulants or stim-laden fat burners, on the other hand, are probably rather counterproductive. Their ability to free fat from the adipose tissue is of no avail, when you are "bulking" - in the worst case it may even repartition the fat from the periphery to the trunk. So you better tick to one serving of stims before a workout.

        10 rules of thumb to help you making HIIT a Hit

        I could probably ramble on forever, but don't think that this will provide any significant insights beyond what I have written in the previous paragraphs. So let's put an end to this endless post and formulate a couple of rules of thumb:
        1. Don't get overtly focused on HIIT there are benefits to steady state exercise, as well; and it goes without saying that you won't be willing to give up weight lifting completely. You can better live without HIIT and just lifting + LISS than with HIIT only.
        2. If you want to improve your conditioning, you will have to train on the higher end of the 15s to 4min interval continuum.
        3. If you want to improve your body composition, training at the lower end of the 15s to 4 min interval continuum and combining shorter HIIT workouts with either strength or aerobic work appear to be more promising.
        4. Active rest should always be preferred over passive rest, regardless of your goals
        5. The length of the rest periods should increase with increasing training intensity
        6. If you are doing HIIT for fat loss, longer active rest (up to 5 min) can actually increase the amount of fat you burn and may be a viable alternative for an additional LISS workout
        7. Creatine, BCAAs, caffeine and baking soda, can be used before a HIIT workout regardless of your training goals
        8. Continuous progression is obligatory, if you want to maximize your conditioning
        9. Stick to the same intense, but short HIIT regimen, when you are dieting in order not to fall victim to the "doing more and eating less downward spiral".
        10. Know your limits and don't simply add hours of HIIT to your current regimen.
        Based on these rules of thumbs and the rest of the information in this installment, as well as the examples in the previous installment you should actually be able to come up with some kind of workout you believe you can benefit from. Give it a two week trial before you decide whether it's too hard, too easy or whatever and don't expect magic results. In the end, HIIT is like a potent supplement: Those who have what it takes to make progress without it will benefit. Those who have stalled on each and every regimen they tried, will stall on hit as well (most likely because they overtrain and/or follow  a crappy diet).

        References:
        • Balsom PD, Seger JY, Sjödin B, Ekblom B. Maximal-intensity intermittent exercise: effect of recovery duration. Int J Sports Med. 1992 Oct;13(7):528-33.
        • Billat V, Binsse V, Petit B, Koralsztein JP. High level runners are able to maintain a VO2 steady-state below VO2max in an all-out run over their critical velocity. Arch Physiol Biochem. 1998 Feb;106(1):38-45.
        • Billat VL, Flechet B, Petit B, Muriaux G, Koralsztein JP. Interval training at VO2max: effects on aerobic performance and overtraining markers. Med Sci Sports Exerc. 1999 Jan;31(1):156-63.
        • Billat LV. Interval training for performance: a scientific and empirical practice. Special recommendations for middle- and long-distance running. Part I: aerobic interval training. Sports Med. 2001;31(1):13-31. 
        • de Souza EO, Tricoli V, Roschel H, Brum PC, Bacurau AV, Ferreira JC, Aoki MS, Neves-Jr M, Aihara AY, da Rocha Correa Fernandes A, Ugrinowitsch C. Molecular Adaptations to Concurrent Training. Int J Sports Med. 2013 Oct 8.
        • Gunnarsson TP, Bangsbo J. The 10-20-30 training concept improves performance and health profile in moderately trained runners. J Appl Physiol. 2013a Jul;113(1):16-24. 
        • Gunnarsson TP, Christensen PM, Holse K, Christiansen D, Bangsbo J. Effect of additional speed endurance training on performance and muscle adaptations. Med Sci Sports Exerc. 2013b Oct;44(10):1942-8. 
        • Laursen PB, Jenkins DG. The scientific basis for high-intensity interval training: optimising training programmes and maximising performance in highly trained endurance athletes. Sports Med. 2002a;32(1):53-73. 
        • Laursen PB, Blanchard MA, Jenkins DG. Acute high-intensity interval training improves Tvent and peak power output in highly trained males. Can J Appl Physiol. 2002b Aug;27(4):336-48.
        • Lindsay FH, Hawley JA, Myburgh KH, Schomer HH, Noakes TD, Dennis SC. Improved athletic performance in highly trained cyclists after interval training. Med Sci Sports Exerc. 1996 Nov;28(11):1427-34.
        • Smith AE, Fukuda DH, Kendall KL, Stout JR. The effects of a pre-workout supplement containing caffeine, creatine, and amino acids during three weeks of high-intensity exercise on aerobic and anaerobic performance. J Int Soc Sports Nutr. 2010 Feb 15;7:10.
        • Stepto NK, Hawley JA, Dennis SC, Hopkins WG. Effects of different interval-training programs on cycling time-trial performance. Med Sci Sports Exerc. 1999 May;31(5):736-41.
        • Stepto NK, Martin DT, Fallon KE, Hawley JA. Metabolic demands of intense aerobic interval training in competitive cyclists. Med Sci Sports Exerc. 2001 Feb;33(2):303-10.
        • Westgarth-Taylor C, Hawley JA, Rickard S, Myburgh KH, Noakes TD, Dennis SC. Metabolic and performance adaptations to interval training in endurance-trained cyclists. Eur J Appl Physiol Occup Physiol. 1997;75(4):298-304.
        • Weston AR, Myburgh KH, Lindsay FH, Dennis SC, Noakes TD, Hawley JA. Skeletal muscle buffering capacity and endurance performance after high-intensity interval training by well-trained cyclists. Eur J Appl Physiol Occup Physiol. 1997;75(1):7-13.

        Thursday, December 19, 2013

        Fat Loss Principles That Work: 10g+ of EAAs W/ Every Meal. Do Energetic Costs of Protein Synthesis Trigger This Effect?

        EAAs beyond whey: It may not necessarily look like this, but this salad (repicecorner) is an EAA power horse with cheddar cheese (25% protein, 0.49 EAA / P ratio), tuna (in oil, 29%,  0.45) and kidney beans (9%, 0.45). You see, it does not always have to be chicken breasts or whey to get beyond the 10g+ EAA threshold, I have repeatedly suggested as one of the fundamental rules of dieting for weight loss, maintenance and muscle gain.
        Many people take it for granted that you become fat, when you get old. If you look at the statistics, you could even make a point that obesity has some protective effects with esp. with respect to CVD mortality. Scientists call this the "obesity paradox" (Kastorini. 2013). What's particularly paradox, at least in my humble opinion, is yet not the phenomenon itself, but rather the fact that it gets smart scientists derailed from working on useful dietary and exercise interventions to prevent the development of heart disease, cancer, metabolic syndrome etc. in early years. Instead, they argue ex-post, i.e. when the baby has already been thrown out with the bathtub by comparing sick lean (in parts even cachectic) and sick "obese" people, why their statistical shenanigan that's based on the useless BMI produces paradoxical results. And that, when studies such as the one Jacobs et al. did in 2010 clearly show that 50+ year old men and women with waist circumference >120cm and >110cm, respectively, have 2x higher all-cause mortality risk than their lean peers - irrespective of BMI (Jacobs. 2010)!

        To get lean and stay lean, yet not thin and skinny fat is therefore a challenge everyone...

        ... from the child in the Kindergarten to the obese granny in the nursing home is facing. Against that background a previous study by Loenneke et al. comes to mind. The results of their analysis, which were published in Nutrition and Metabolism in January 2013 clearly show that the amount of times people eat meals with a 10g+ EAA content per day was inversely related to percent central abdominal fat (Loenneke. 2013). In previous studies EAAs have also been shown to improve glucose clearance without increases in insulin and in the absence of effects on the fat burnin and health promoting expresion of AMPK-alpha2 in skeletal muscle tissue (see "EAAs Stimulate Muscle Glucose Uptake by Exponentiating Insulin's Effect on GLUT4 Expression"). With the advanced publication of a study by Coker, Miller, Schutzler, Deutz and Wolfe in the online verison of the Nutrition Journal a couple of days ago, the notion that EAAs have a particularly beneficial effect on fat loss - in this case in obese elderly individuals - gets further support from a well-controlled randomized trial (Cooker. 2013).

        EAA-rich protein increases fat loss to a greater extent than low EAA protein

        The researchers from the Center for Translational Research in Aging and Longevity and the University of Arkansas for Medical Sciences in Little Rock, AR, USA randomized 12 elderly individuals (mean age 69 years) to an 8 week, caloric restriction diet utilizing equivalent caloric meal replacements (~850 kcal/day; the exact nutrient composition can be found in figure 1) + ~400kcal from solid foods (total intake: ~1,250kcal/day; the subjects were free to chose their solid meals but were provided with a list of examples the should pick from, if possible).
        Figure 1: Macronutrient composition of the meal replacements used in the study (Cooker. 2013)
        The diet was designed to induce a 7% weight loss in two months. And while both,  the rate of weight loss (~1.6lbs per week), as well as the relatively high caloric deficit are certainly appropriate for someone with a 30+ BMI and ~40% body fat, leaner people will fare better with a less pronounced kcal deficit or (alternatively) have to add some strategically planned refeeds to the equation in order to minimize the loss of lean mass and, more importantly, avoid the ensuing reduction in energy expenditure (for the obese, the latter is actually less of the problem, because the downsides of being calorically deprived are at least partly counglucose tolerance and leptin sensitivity with every gram of body can actually help the body recognize that there is still plenty of energy that has just not been available (glucose) or "visible" (fat) before).
        Figure 2: Changes in lean and fat mass (kg, left) and fractional protein synthesis rates (FSR) in participants receiving iso-caloric meal replacements with identical macronutrient compositions (see figure 1), but different amounts of essential amino acids (EAAs) content (Coker. 2013)
        As the data in figure 2 goes to show you even the obese individuals in the study at hand lost a non-negligible amount of lean mass - unfortunately the body composition was measure with a sophisticated, but still body impedance based device, the trends are still accurate, but it is questionable in how much we are actually talking about ~2 and 2.5kg of muscle mass (figure 2, left), because somebody's "lean body mass" does obviously include more than just skeletal muscle.

        When it comes to supplements, we are often like children on Christmas eve. About all the new stuff we get we tend to forget our former favorite and often way more fun to play with toys. Don't make this mistake and ditch your PWO whey (personally, I like a ~1.5:1 whey + micellar casein mixture) for EAAs, they don't come close... read more
        Be that as it may - since the before and after values were taken with the same device the changes should be correct, so that both the slightly yet not statistically significantly ameliorated loss of lean body mass and, more importantly, the significantly higher degree of body fat loss in the EAA meal replacement (EAAMR) group speak in favor of the 5 servings of a the 170 kcal, 6g EAA per day. Moreover, "the sparing influence of muscle loss might have been demonstrated with a larger sample size", so that you can take it for granted that the preservation of precious muscle mass is an advantage of being choosy with your protein sources and preferring those with higher over those with lower essential amino acid contents.

        On a related note: I don't know if you noticed, but with a total energy content of 850kcal and 30g EAA these 5 meal replacements did in fact have exactly those 10g+ of essential amino acids, I have repeatedly recommended to have with each of the 3 meals most people consume in the course of the day.

        In all fairness, it should also be mentioned that despite not being significantly different at baseline, the body fat percentage of the subjects in the EAA meal replacement group was ~3% higher to begin with.This may seem irrelevant, since figure 2 compares lean mass and fat mass as absolute changes and not their percentages, but in the end, the amount of fat you you can drop within a given time-frame decreases with lower body fat percentages.

        Do the energetic costs of protein synthesis drive fat loss?

        Another interesting observation Coker et al. made is the close association between fat loss, on the one hand, and increased protein synthesis (55%), on the other hand. The researchers take this as an incentive to do one of the of the much loved calories in vs. calories out calculation and come up with the following hypothesis:
        "Acute administration of EAAMR did promote a significant increase in skeletal muscle protein FSR compared to CMR. Assuming that the energy cost of protein synthesis is 3.6 kJ/g and the baseline GAIA-derived lean tissue mass was 56.4 kg for EAAMR and 54.4 kg for the CMR, we can extrapolate that the overall energy discrepancy between the two groups was roughly equivalent to 27,170 kcal or 3.5 kg of weight loss across the entire caloric restriction-based weight loss paradigm. Based on the amount of total lean mass in each group, this value takes into account a consistent intervention structure of five servings/day across an eight week period. In short, these calculations suggest that differences in the source of intact protein/formulation of EAA may have a significant influence on diet-induced energy expenditure that coincides closely with the greater reduction of adipose tissue in EAAMR compared to CMR." (my emphasis in Coker. 2013)
        I usually discard fallacious calculations like this one if they are not highlight the stupidity of trying to eat exactly as much as some funky formula + the figure on your treadmill, pedometer, heart rate monitor or whatever fancy tool you may use to "measure" your energy expenditure suggest you would have burned in the last 24h. In this case, however, I made an exception, because I feel that the notion that protein quality is one of the myriad of parameters that are missing from this foolish calculation is important, for lean and obese people from all age groups who are trying to shed body fat.

        Bottom line: The take away message of the study is in the end identical to the previously mentioned study by Loenneke et al.: Make sure you hit the 10g EAA threshold with each and every of your meals, if being lean and muscular not skinny yet fat is your goal.

        References:
        • Coker RH, Miller S, Schutlzer S, Deutz N, Wolfe RR. Whey protein and essential amino acids promote the reduction of adipose tissue and increased muscle protein synthesis during caloric restriction-induced weight loss in elderly, obese individuals. Nutr J. 2013 Dec 11;11(1):105. [Epub ahead of print]
        • Jacobs EJ, Newton CC, Wang Y, Patel AV, McCullough ML, Campbell PT, Thun MJ, Gapstur SM. Waist circumference and all-cause mortality in a large US cohort. Arch Intern Med. 2010 Aug 9;170(15):1293-301.
        • Kastorini CM, Panagiotakos DB. The obesity paradox: methodological considerations based on epidemiological and clinical evidence--new insights. Maturitas. 2013 Jul;72(3):220-4.
        • Loenneke JP, Wilson JM, Manninen AH, Wray ME, Barnes JT, Pujol TJ. Quality protein intake is inversely related with abdominal fat. Nutr Metab (Lond). 2013 Jan 27;9(1):5.

        Monday, December 16, 2013

        Making HIIT a Hit Part I/II: The Quest for the Optimal Interval to Rest Ratio for Your Type & Goals - Warming up: Selected Studies + Individual Take Home Messages to Set the Scene

        This series will not readdress the "HIIT and/or LISS debate", but simply try to do what the title says: Help you to determine the "ideal" form of HIIT for your type & goals.
        After posting yet another article about the benefits of high intensity interval training (HIIT) training on Wednesday, I thought it may be a good idea to take a look at the pertinent literature to get a better grasp of what exactly high intensity interval training actually is... "What it is? What do you mean?" While almost everyone appears to recommend you do at least some amount of high intensity interval training, these days, the definition of both the "high intensity" and the "intervals" which are more than just eponymous of this type of training are usually pretty wooly. The two questions this article is supposed to answer are therefore: How long should the intervals and rest periods be and at which intensity should they be performed.
        Attention, this article turned into a two part series: I must warn you, this "quest" turned out to be hell lot of work and way more than I had expected. So I will have to split it up into a two-part series. With today's first part discussing exemplary "success stories" and the subsequent follow up on next Sunday providing a more comprehensive overview and general conclusions one may draw based on the picture that emerges.

        Part I: Success stories - learning from the "best"

        I decided that it may be a good idea to initially compile some examples of which you could say that they are examples of a successfully implementation of high intensity interval training. In that, I will put an emphasis on "old" studies that have not yet been discussed on the SuppVersity. This means, if you want more examples before next week's 2nd installment with a more general overview, you can simply click through the archive of articles that are marked with the keyword HIIT - either as HTML or RSS (I recommend the latter if you do not intend to read all of them anyway; opens in all good browsers without a reader).

        Untrained participants and non-athletes
        • 5min on, 2min off HIIT to increase fitness (VO2max) alternated with 40 min steady state cardio for 4 + 5 weeks (Hickson. 1981) -- Hard to believe but it's more than 30(!) years ago that Hickson, Hagberg and Ehsani were able to show that a combination of high intensity interval training with 6x5min cycling at 90-100% of the VO2max and 2-min at 30%-50% active rest between intervals on day one and 40 min of steady state "cardio" on a treadmill on day 2 (6 workouts per week) yielded profound increases in VO2max:
          Figure 1: Adaptation to combined HIIT + steady state protocol in "occasionally active" but not "regularly" trained men (n=8) and women (n=1) in the Hickson study (Hickson. 1982).
          For our purpose, the most significant finding of this study may however be that the t1/2, i.e. the amount of time it takes for 50% of the adaptations to take place is only 10 days (on this intense protocol).

          Take home message: If VO2Max and overall conditioning is your goal you should up the intensity every three weeks, to make sure you continue to make progress. If you go by VO2max, this would be an increase in the speed you run or bike - not (!) the duration of the intervals and / or their number.

        • 2 series of 5s sprint cycling (8-13x) with 55s of active rest increase force production and rids subjects of "useless" type IIb fibers (Linossier. 1993) -- The 10 students (8 men, 2 women; age 22y; VO2max = 51.2ml/kg/min, everything below 55 is still considered ) in the Linossier study performed 4 HIIT only workouts consisting of 5s all-out sprints on a cycle ergometer interspersed by 55s of active rest cycling at a heart rate of 130-140bpm per week. The number of sprints in each of the two sessions on a single training day (15 min of rest between) were increased from 8 to 13 sprints over the 7-week study period. The main results were improvements in both peak performances +25%  and in the 30-s total work +16%.

          Figure 2: Unexpected shift in muscle fiber type distribution (Linossier. 1993)
          What's also relevant, though not directly performance related is the 19% increase in phosphofructokinase and a 20% increase in lactate dehydrogenase, both are glycolytic enzymes and are indicative of improvements in the glycolytic pathway, which goes to show you that you don't have to be afraid of the fiber type changes hampering either your ability to handle glucose or your strength - I mean +29% in maximal force production in the sprint tests are everything else but "weak".

          Take home message: Shorter sprints appear to be a valid means to hammer and improve the glycolytic pathway and get rid of the useless type IIb fibers, of which not endurance athletes, but bodybuilders have the lowest amount and sedentary controls the most (cf. figure 1 in the overview of the Intermittent Thoughts on Building Muscle). To actually build muscle the 1% increase in muscle weight the scientists observed, is yet not sufficient enough.

        • 7 sessions of 10x4 min cycling at 90% with 2 min rest ramp up skeletal muscle fat metabolism in young women (Talanian. 2006) -- The 8 healthy recreationally active women (22 yr old, 65.0 kg body wt) who had previously engaged in 2-3 just-for-fun sessions of various activities from weight lifting, soccer and cycling to swimming, or walking, participated in no more than 7 HIIT sessions within 2 weeks (1 day on, 1 day off) which consisted of 10x 4min sprints at 90% of their individual VO2max with two minutes of rest in between.

          Figure 3. Changes in substrate utilization during standardized 60 min LISS test at 60% of the VO2Max (Talanian. 2006)
          Contrary to the previously discussed studies, the scientists were in this case less interested in the performance than in the metabolic benefits, which were - as the data in figure 1 show - surprisingly significant given the short duration of the study. The increases in fat oxidation and decreases in the respiratory ratio (the ratio of glucose to fatty acid oxidation) during a standardized 60min light intensity (60% VO2max) test that was performed at the beginning and end of the study, were a consequence of increases in citrate synthase and beta-hydroxyacyl-CoA dehydrogenase (beta -HAD) activity.

          Take home message: In view of the important contribution of beta-HAD to the beta oxidation of (esp. medium-chain) fatty acids, the fact that an upregulation of this enzyme has been observed in response to the long duration intervals, but neither light intensitsy exercise (even w/ 2h per day, 5-6 times per week; cf. Phillipps. 1996), nor during a similar 2-week sprint training protocol (30s all-out on cycle ergometer, 4 min rest; cf. Burgomaster. 2005) points towards "long" duration intervals when mitochondrial adaptations that favor fatty oxidation and thus subsequent increases in fat loss with light activity are concerned. 

        Highly trained participants and pro-athletes
         

        • 3 sessions of 10 intervals at 96% VO2Max with 60-180s rest increase running economy and relative fatty oxidation during subsequent steady state exercise test (Zavorsky. 1998) -- Twelve highly trained endurance athletes volunteered for this study, four of them qualified for the 1996 Canadian Olympic Trials, and one subject was a Canadian record holder in triathlon. On three different occasions the subject ran 10 x 400-m sprints with active recovery periods of 60, 120, 180s (randomly assigned). 10 min before and afterwards standardized running economy tests were performed.

          Figure 4: Respiratory exchange ratio in RE test before and after the sprinting protocol (Zavorsky. 1998)
          During these running economy tests, which consisted of 2 x 5 min running on a treadmill at 12km/h or 16km/h (5 min total rest in between), the subjects exhibited, much contrary to the research hypothesis, by the way, an increase in the running economy (RE, a measure of how efficiently a person uses oxygen while running at a given pace) and - probably of greater interest for most of you an increase in fatty acid oxidation, as evidenced by the reduced respiratory exchange ratio (RER, figure 4). The latter was particularly pronounced in the "low intensity" RE test at 12km/h.

          As far as the differential effects of the rest intervals is concerned, significant effects were observed for the rates of perceived exertion (60s: 17.7; 120s: 16.1; 180s: 14.4), but not for the performance related measurements velocity, and the time it took the athletes to run the 400m sprints (actually the latter should be self-evident with identical velocities).

          Take home message: Doing ten short sprints (if you are not an athlete, shorter ones will suffice) before you go jogging (or do any other type of steady state cardio) won't hamper your running economy. And since the study at hand suggests that the relative increase in fatty acid oxidation is specifically pronounced in the lower intensities (this is supposedly even more the case if you jog at 10km/h), the combination of sprints + steady state cardio appears to be an ideal "cardio" only day, when you are trying to lose body fat.

        • 8x all-out (100% VO2max) 2.5min intervals with 4 min of active rest increase VO2Max, peak aerobic power and 5k time trial performance regardless rest between sessions (Gross. 2007) -- With the unique twist with respect to the training frequency this study is somewhat unique. While other studies report similar benefits in highly trained collegiate cyclists (13 men, 4 women; VO2Max at baseline 62ml/kg/min) or other endurance athletes, this is one of the few that investigates whether it makes a difference if the athletes implement the 3-days per week HIIT regimen as a block or interspersed by one day of rest into their regimen.

          Percent change in TT5k velocitym TT5k power output VO2peak, and peak aerobic power output in cyclists trainin on consecutive vs. non-consecutive days (Gross. 2007)
          Interestingly enough, neither the performance outcome on the pre- and post tests, i.e. increases in VO2Max (+5.7%), peak aerobic power (+7.2%), 5k time trial performance (+6.9%), nor the actual performance during each of the workouts the subjects performed in the course of the 3-week study period suffered from doing the HIIT sessions back to back. These results refute previous speculations that doing HIIT as a block would trigger greater metabolic adaptations, specifically in athletes (e.g. Padilla. 2000). Even more, though the changes were not statistically significant, the data in figure 5 shows that - with one for endurance athletes important exception, namely the VO2Max - training on non-consecutive days produced marginally better results.

          Take home message: While it appears as if it would not make a difference whether you perform your HIIT workouts blocked or within your training week. The non-significant differences in figure 5 could suggest that endurance athletes intending to improve their already high VO2Max even further would be better off with the blocked training. Everybody else has the choice and I would pick the interspersed variety, whenever my schedule allows me to do this - my personal experience told me that this works better for me... apropos, take another look at figure 5 you see the narrow bars indicating the standard deviations? This goes to tell you that it is very likely that not just the personal preferences, but also the actual outcome will vary from trainee to trainee, which supports the notion that you will have to experiment to find what works best for you.

          That's it for the "success stories"! Don't forget to come back next Sunday for part II and in case you have not done so already, just browse the previous SuppVersity posts on this matter, either as HTML version, post by post, or from the RSS overview (works in every modern browser, yet not in Google spyware ;-). I know you are smart enough to draw your own conclusions. And what's more, this may yield some cognitive input for the comment area of this article, where you can post questions. I will try to tackle those I can answer in the next installment.

              References:
              • Burgomaster KA, Hughes SC, Heigenhauser GJF, Bradwell SN, Gibala MJ. Six sessions of sprint interval training increase muscle oxidative potential and cycle endurance capacity in humans. J Appl Physiol. 2005; 98: 1985–1990.  
              • Gross M, Swensen T, King D. Nonconsecutive- versus consecutive-day high-intensity interval training in cyclists. Med Sci Sports Exerc. 2007 Sep;39(9):1666-71.
              • Hickson RC, Hagberg JM, Ehsani AA, et al. Time course of the adaptive responses of aerobic power and heart rate to training. Med Sci Sports Exerc 1981; 13: 17-20.
              • Linossier MT, Denis C, Dormois D, Geyssant A, Lacour JR. Ergometric and metabolic adaptation to a 5-s sprint training programme. Eur J Appl Physiol Occup Physiol. 1993;67(5):408-14. 
              • Padilla S, Mujika I, Orbañanos J, Angulo F. Exercise intensity during competition time trials in professional road cycling. Med Sci Sports Exerc. 2000 Apr;32(4):850-6.
              • Phillips SM, Green HJ, Tarnopolsky MA, Heigenhauser GJ, Grant SM. Progressive effect of endurance training on metabolic adaptations in working skeletal muscle. Am J Physiol Endocrinol Metab. 1996: 270: E265– E272, 1996
              • Talanian JL, Galloway SD, Heigenhauser GJ, Bonen A, Spriet LL. Two weeks of high-intensity aerobic interval training increases the capacity for fat oxidation during exercise in women. J Appl Physiol. 2007 Apr;102(4):1439-47. 
              • Zavorsky GS, Montgomery DL, Pearsall DJ. Effect of intense interval workouts on running economy using three recovery durations. Eur J Appl Physiol. 1998; 77: 224-30.