Sunday, November 24, 2013

Get Lean & Stay Lean Quickie: OTC Fat Loss Supps Under Scrutiny. Sleepless Yet Lean in India?! Sesamine - Falsely Forgotten? High Carb Nighttime Snacks for Fridge Raiders?

Without an optimized dietary routine, a sound training plan and tons of discipline no fat burner is going to get you abs like these (suggested read: The SBSG Fat Loss Support Routine)
I suppose by today, the last remnants of your turkeys should be gone and you and your relatives ~0.5kg heavier (Hull. 2006). Against that background it appears only logical to turn this week's installment of On Short Notice into another Get Lean & Stay Lean Quickie. Moreover, with the 0.5kg the average American gains in the course of the Thanksgiving holidays, I already have my (or should I say your? Be honest ;-) figure of the week, so that there is actually no reason why we could not dive right into the science of fat loss.

As you are about to see, this installment has more ineffective than effective fat loss treats. Why? Well, maybe due to the fact that it harbors two studies on commercially available supplements? But whom am I telling this... you already know that the main benefits of these so-called "thermogenics" are actually related to their appetite suppressing and stimulating effects, which can help you stick to your diet and workout regimen, and not to their ability to actively "burn" body fat.

Apropos stimulant: Were you aware that there could be methamphetamine in a common ingredient of some thermogenics? No? I'd suggest you check out the last news of this Get Lean & Stay Lean Quickie first, then. If you are not interested in "scandalous" revelations of which you don't even know if they have a bearing on the extracts that are used in your favorite Acacia rigidula supplement (I would actually hope you don't have one, but anyway), you may obviously start at the top, as well:
  • Weight loss stack fails to produce results: Caffeine + BCAA + CLA + Green tea = soy bean oil placebo (Thomas. 2013) At the 9th Annual ISSN Conference and Expo, Daniel Thomas and his co-workers  presented a study in which they investigated the effects an commercially available multi-ingredient dietary supplement containing 99mg of caffeine and a proprietary blend containing 1510 mg of CLA, green tea extract (45% EGCG), L-leucine, L-iso-leucine and L-valine in 22 obese volunteers (placebo arm: age, 34 ± 12; BMI, 34.1 ± 6.1; active arm: age, 36 ± 11.1 years; BMI, 30.0 ± 4.9).

    The supplement / placebo had to be taken with breakfast and lunch (with two pills per serving this would amount to 400mg of caffeine per day and an undisclosed amount of CLA, green tea and BCAAs, of which you can yet probably safely assume that they were underdosed). Body composition and android fat (dual-energy X-ray absorptiometry), waist and hip circumferences, blood pressure and heart rate were measured at baseline and after 8 weeks of supplementation. Aside from taking the supplement the participants were advised to stick to their regular dietary and activity patterns. Against that background it is not exactly surprising that the 'wonder pills' did not bring about any changes in body composition, android fat, waist or hip circumference; and in contrast to the acute effects of the  "hardcore" competition about which you can read in the last post of today's Get Lean & Stay Lean Quickie the product did not even increase the heart rate and blood pressure of the subjects.
  • Blood sugar levels of Indian adolescents are not associated with insufficient sleep and yet not sleeping enough still takes its toll (Patel. 2013) -- You've read more than enough about the importance of sleep on the SuppVersity within the past couple of months (e.g. The SuppVersity Circadian Rythm Series) to be surprised by what Patel et al. conclude in the abstract of  their latest paper:
    "The current study indicates that inadequate sleep duration at night (<7 hrs) does not affect the blood glucose level of the Gujarati Indian adolescents of age group 13-20 years." (Patel. 2013)
    Unfortunately, this is yet again an instance where cursory skimming the abstract provides a  skewed image of the actual study results, which - as you can see in my plot of the actual results - did very well confirm previous observations of the same authors and the findings of the majority of studies which investigated the effects of insufficient sleep on body composition in Western adolescents.

    Figure 1: Body fat (%), fat free mass (FFM) and waist circumference in Indian adolescents (Patel. 2013)
    Statistically significant were the respective differences only in the male part of the study population. That's yet probably just a result of the low number of female participants which was not only significantly smaller (N=95 vs. N=237), but also very unevenly distributed as far as the ratio of female adolescents with adequate (N=90) and inadequate sleep (N=5) are concerned. Against that background you should also exert some caution with respect to the fat free mass values in the N=5 short sleepers. It would probably suffice to have one muscular athlete in this group to skew the whole results.

    Apropos "short sleepers": Can you imagine that only 14% of the male and 5% of the female Indian Gujarati adolescents actually didn't get their share of 7h+ sleep per day!? Makes me wonder about the number of smartphones, Playstations and cable TV channels in the Anand district where the 332 Gujarati adolescent school and and college students came from - the same goes for the respective interactions between nutrient quality, sleep duration and family income.
  • Study shows addition of fish oil accentuates sesamin's 'fat burning effects' (Ide. 2013b) In what could be considered a follow up to the results of a previous study in which Ide et al. were able to show that the addition of arachidonic acid (ARA) to sesamin supplemented chow augmented body fat loss, and increased the expression of enzymes that are involved in the oxidation of fatty acids, Takashe Ide has just published another study, which shows that high dose fish oil (15-20g/kg chow) will illicit similar, if not even more pronounced effects on the expression of Carnitine palmitoyltransferase 2, which is necessary to transports fatty acids into the mitochondria, and the alpha and beta subunit of the trifunctional enzymes that are involved in their subsequent oxidation.

    Figure 2: Effects of Sesamin (SES) + fish oil or arachidonic acid (ARA) on mRNA expression of CPT, trifunctional enzymes alpha & beta, as well as serum lipids in rodents after 15/16 days on respective diets  (Ide. 2013a & 2013b)
    As the data in figure 2 goes to show you, these increases were accompanied physiologically relevant decreases in the concentrations of triglycerides, cholesterol and phospholipids in the blood of the 5-week old male Sprague Dawley rats, Ide used in his latest study. Moreover, the observation that the supplementation regimen enhanced increases in mRNA of the peroxisomal enzymes involved in fatty acid oxidation and
    a membrane protein (peroxin-11α) associated with peroxisomes without affecting enzymes associated with mitochondria and microsomal cytochrome P-450 4a1 expression indicates the "existence of a mechanism independent of PPARα to specifically induce the gene expression of peroxisomal proteins." (Ide. 2013b)  That's an unquestionably interesting observation; also because it could open up new avenues for the development of anti-diabesiety drugs or the identification of herbs and natural "fat burners".
  • More scientific evidence: Eating carbs in the evening does not necessarily make you fat - even if you eat them instead of protein! (Eddy. 2013) While I would still be hesitant to recommend the ingestion of maltodextrin instead of casein or whey protein as a pre-bed snack, the results of a recent study by Eddy et al. clearly show that the sugar load right before bed did not have negative effects on the 59 sedentary, overweight and obese volunteers who were randomly assigned to ingest isocaloric amounts of maltodextrin (PLA), casein (CAS) or whey (WP) max. 30min before bed.
    Carbs Before Bed: What's good for overweight Israeli police cannot be bad for overweight Americans, can it? (photo by Mark Probst)
    "No significant group differences existed at baseline. There were no group x time interactions for RMR, hunger, satiety, desire to eat, fat mass, lean body mass, or weight (P< 0.05), although RMR displayed a trend towards significance with the PLA group decreasing by 74.3 ± 94.5 and WP and CP increasing by 235.73 ± 84.5 and 51.7 ± 79.4kcal/day, respectively (P=0.0559). Significant time effects were measured for satiety (pre: 31.5 ± 2.3, post: 40.6 ± 2.3, P< 0.008) and LBM (pre: 51.8 ± 0.1, post: 52.3 ± 0.1, P< 0.0001)." (Eddy. 2013)
    In view of the fact that it cannot be said if the absence of negative effects on hunger, satiety, desire to eat, fat mass, lean body mass, or weight was related to the obligatory supervised exercise sessions (3x/week; 2 days of resistance exercise and 1 day of high-intensity cardiovascular exercise) all participants had to attend, it does yet remain to be seen if similar results would be observed in obese (or lean?) subjects in the absence of a supervised resistance training and HIIT workouts. That said, as "non-significant" as the previously cited trend in resting metabolic rate ((RMR) may be, the increase in the amount of energy the obese subjects spent sitting around in both protein groups and the contrasting decrease in the maltodextrin group, is something to keep in mind .
    Ok, nighttime snacking increases LDL, but if you measure it in the morning after a high carb + high fat  snack that alone can contribute to the statistically "significant", but physiologically irrelevant LDL increase of 7mg/dL the scientists observed in their 11 healthy participants.
    Midnight snacking (Hibi. 2013): Whether eating right before bed is a good idea at all was not addressed in the study at hand and according to an even more recent paper by Hibi et al., postponing your 10am 192kcal snack (mean protein : fat : carbohydrate ratio of 5:50:45) to 11PM will significantly decrease fat oxidation (daytime snacking: 52.0 ± 13.6 g/d; nighttime snacking: 45.8 ± 14.0 g/d; P = 0.02) and increase total and LDL cholesterol significantly. How bad that actually is, is however likewise questionable, after all the blood glucose and insulin levels, snack and total energy intake, body weight, and energy expenditure of the 11 healthy women (age: 23±1 y; body mass index: 20.6 ± 2.6 kg/m²) who participated in the randomized-crossover trial were not affected by the switch from the daytime to the nighttime snack.
    Irrespective of any trends and non-significant differences, eating a heap of pure sugar (~35g - this is based on the assumption that the amounts were identical to another recent study by the same group which tested the acute effects of carbs and protein, cf. Kinsey. 2013) before going to bed has little to nothing to do with having a whole meal, with or without carbohydrates before bed -- and that this can increase not hamper weight loss, is something you as a SuppVersity reader are well aware of (see "Carbs after 6PM Will Make You Lean" & "Carbs After 6PM Reloaded").
  • Figure 3: According to a somwhat dubious study Acacia rigidula contains more than 44 "toxic amines and alkaloids" (data in ppm; Clement. 1998)
    Hi Tech Pharmaceuticals sponsored trial finds: Fastin RX is better than only two of its ingredients (Jacobs. 2013) -- I guess you won't be surprised to hear that the addition of  methlsynephrine, 1,3 dimethylamylamine ("geranium extract"), yohimbine HCL, naringen and theobromine to caffeine and Acacia rigidula extract potentiates the effects of either 300 mg caffeine (C) or 250 mg Acacia rigidula (AC) alone or in combination, right?

    Fine, 'cause this leaves more room for the important question, whether an additional increase in heart rate of 6.9 and 6.0bpm 2h and 3h after the ingestion of the extended release "fat burner" Fastin RX, a significant increase in systolic blood pressure of 33%, 26% and 19%, as well as increases in diastolic blood pressure that were 16.6%, 2.9% and 15% higher than in the caffeine (only) trial have any bearing on the efficacy of this product, when the 10.1%, 10.0% and 4% greater VO2 consumption (compared to AC and C, only) in the first three hours after the ingestion of the diet pill did no not show any significant main or interaction effects on resting metabolic rate? Probably not? Yeah... I would guess so, as well.
    That's it for today... aside from the advice not to freak out over whatever amount of weight you may have gained in the past couple of days, I shold say: Trust me, it's not worth stressing yourself this is just going to make things worse. Just return to your regular nutritional habits, keep working out and it will be gone in no time. I'd also suggest you check out the latest  SuppVersity Facebook News on
    • Aqueous dried barberry extract as a treat for acne vulgaris (learn more)
    • Zinc + phytoestrogens vs. osteoporosis - a double- yet dull-edged sword  (learn more)
    • Cold-water immersion beats passive recovery and contrast water therapy for recovery after an intense American football training (learn more)
    • Study from the Boston University School of Medicine says: Female adolescents don't eat enough meat. (learn more)
    and the other news I posted today and am going to post in the course of the next 24h. I guess that should suffice to bridge the time until I post tomorrow's full-length SuppVersity article. I'll see you tomorrow, then!

    References:
    • Clement BA, Goff CM, Forbes TDA. Toxic amines and alkaloids from acacia rigidula. Phytochemistry, Volume 49, Issue 5, 5 November 1998, Pages 1377–1380. 
    • Hibi M, Masumoto A, Naito Y, Kiuchi K, Yoshimoto Y, Matsumoto M, Katashima M, Oka J, Ikemoto S. Nighttime snacking reduces whole body fat oxidation and increases LDL cholesterol in healthy young women. Am J Physiol Regul Integr Comp Physiol. 2013 Nov 21.
    • Hull HR, Hester CN, Fields DA. The effect of the holiday season on body weight and composition in college students. Nutr Metab (Lond). 2006 Dec 28;3:44.
    • Ide T, Ono Y, Kawashima H, Kiso Y. Interrelated effects of dihomo-γ-linolenic and arachidonic acids, and sesamin on hepatic fatty acid synthesis and oxidation in rats. Br J Nutr. 2013a Feb 28:1-14.
    • Ide, T. Fish oil at low dietary levels enhances physiological activity of sesamin to increase hepatic fatty acid oxidation in rats. Journal of Clinical Biochemistry and Nutrition. 2013b; 51(3):241–247.
    • Jacobs PL. Acute physiological effects of the commercially available weight loss/energy product, Fastin-XR®, in contrast with the individual effects of caffeine and acacia rigidula. Journal of the International Society of Sports Nutrition 2013, 9(Suppl 1):P10.
    • Kinseyet al.: The effect of acute ingestion of a protein beverage consumed late in the evening on metabolism, appetite, mood state, and blood lipid in overweight and obese adults. Journal of the International Society of Sports Nutrition. 2013; 9(Suppl 1):P16.
    • Patel MC, Shaikh WA, Singh AS. Association of sleep duration with blood glucose level of gujarati indian adolescents. Indian J Physiol Pharmacol 2013; 56(3):229–233.
    • Thomas DD, Rawal S, Kinsey AW, Eddy WE, Fisher N, Spicer MM, Ormsbee MJ. The combination of green tea, caffeine, conjugated linoleic acid and branched chain amino acids have no effect on body composition and abdominal fat changes in overweight and obese men and women. Journal of the International Society of Sports Nutrition. 2013; 9(Suppl 1):P29

    Saturday, November 23, 2013

    Chronic High Dose BCAA Supplementation Reduces Endurance Performance by 43% Plus: How Ammonia, Glutamine, Arginine & Low Carb Could be Involved

    Tired, exhausted, had to cut your workout short today? Is it the flu, or just too much BCAAs?
    When some is good and more is better, even more is not necessarily going to be 'betterer' - and that's not simply due to the fact that there is no comparative to an adjective that's already in the comparative. Therefore it is actually not surprising that a team of researchers from the Department of Food and Experimental Nutrition at the Faculty of Pharmaceutical Sciences, the Department of Nutrition at the School of Public Health and the Department of Physiology and Biophysics at the Institute of Biomedical Sciences of the University of Sã o Paulo in Brazil has just published the results of a study (Falavigna. 2013) which demonstrates that there is an upper limit to the benefits of BCAA supplementation. What I guess will be surprising at least for some not so regular SuppVersity visitors, is that there is more than just a saturation effect: Too much BCAAs can actually have ergolytic (= anti-ergogenic) effects - at least under certain circumstances.

    Another chapter in the book of good things that turn against you, when taken in excess

    In their latest paper that has just been published in nutrients, Gina Falavigna and her colleagues analyzed effects of chronic BCAA supplementation on exercise performance in male Wistar rats. Based on previous animal and human data and the still widely supported, though actually experimentally non-validated (cf. Meeusen. 2007) theory that BCAAs would work their non-hypertrophy specific, endurance enhancing magic via the blockade of exercise induced 5-HT (serotonin) accumulation in the brain, the researchers speculated that ...
    "[...] chronic BCAA supplementation (through the diet, using different BCAA  concentrations) would increase performance in rats subjected to a swimming exhaustion  test." (Falavigna. 2013)
    To verify this hypothesis, Flavigna et al. randomized their rats to three different groups receiving either the standard AIN-93M diet for the maintenance of adult rodents (control group) or the same diet with additional additional 3.57% (group S1) and 4.76% (group S2) BCAAs at a ~2:1:1 ratio of lecine : valine : isoleucine (the BCAAs were manufactured by the Brazilian branch of Ajinomoto). The rodents in the S1 and S2 groups did thus receive 50% and 100% more branched-chain amino acids than the rodents in the control group which had to contend themselves with the BCAAs in the casein fraction of their diets (see figure 1, right). In order to assure that the diets would be isocaloric, an amount of starch equivalent to the amoung of BCCAs that had been added to the chow was removed from the supplemented diets.

    Overall, the study lasted for six weeks. During this time the rodents were subjected to a 1h/day weight bearing swimming protocol five times a week. In the first two weeks, the rats were ...
    "[...] adapted to the water medium and exercised with increasing overloads attached to the tail until an overload corresponding to 5% of total body weight was reached. This final overload was used until the end of the training protocol [...] The overloads were corrected weekly according to the variations in animal weight.  The efficiency of the training protocol was assessed on the basis of maximum activity of the enzyme citrate synthase in the soleus muscle, with a group of sedentary animals being used as the control for this parameter." (Falavigna. 2013)
    Neither the overall amount of food nor the body weight gain of the rodents in the control, and the two exercise groups showed any statistically significant difference. The latter cannot be said about the exercise performance, as well as the accumulation of ammonia, though (see figure 1):
    Figure 1: Exercise duration and plasma ammonia levels during / after swmming test (left) and macronutrient composition of the experimental diets (right; based on Falavigna.. 2013)
    While the rodents in the +50% BCAA group (S1) do show the expected increase in endurance (+37%) their peers in the high dose (+100%) BCAA group (S2) experienced an even more pronounced drop in endurance performance (-43% vs. control), which went hand in hand with a profound increase in blood ammonia (+34%).
    "Ammonia is a ubiquitous metabolic product producing multiple effects on physiological and biochemical systems. Its concentration in several body compartments is elevated during exercise, predominantly by the increased activity of the purine nucleotide cycle in skeletal muscle. Depending on the intensity and duration of exercise, muscle ammonia may be elevated to the extent that it leaks (diffuses) from muscle to blood, and thereby can be carried to other organs. The direction of movement of ammonia or the ammonium ion is dependent on concentration and pH gradients between tissues. As such, ammonia can also cross the blood-brain barrier, although the rate of diffusion of ammonia from blood to brain during exercise is unknown. It seems reasonable to assume that exhaustive exercise may induce a state of acute ammonia toxicity which, although transient and reversible relative to disease states, may be severe enough in critical regions of the central nervous system (CNS) to affect continuing coordinated activity. Regional differences in brain ammonia content, detoxification capacity, and specific sensitivity may account for the variability of precipitating factors and latency of response in CNS-mediated dysfunction arising from an exercise" stimulus, e.g., motor incoordination, ataxia and stupor. There have been numerous suggestions that elevated ammonia is associated with, or perhaps is responsible for, exercise fatigue, although evidence for this relies extensively on temporal relationships." (Falvigna. 2013; my emphasis)
    Mark the last words of the previously cited paragraph: "[E]vidence for [the role of ammonia] in exercise fatigue relies extensively on temporal relationships". It is thus - as for now - a solely corollary, not yet a causative association, of which I do however feel that it would be very likely to turn into a causal one if someone actually measured the influx of ammonia into the brain during a workout.

    Wait, ammonia? But ain't it more likely that the BCAAs block the uptake of tryptophan?

    What's for sure is that another hypothesis, which relates to the blockade of tryptophan uptake can be ruled out as an underlying reason of the differences. After all the scientists who argue that ...
    "[t]he increased synthesis of serotonin during exercise may be related to the development of central fatigue, because this neurotransmitter has several physiological functions, since it operates by  mood, lethargy, individual behavior, regulation of sleep, body temperature and blood  pressure, appetite suppression and changes in perceived exertion." (Falavigna. 2013)
    ...actually measure the 5-HT levels and observed no differences between the dietary groups. Overall, the study results to thus clearly indicate that both, medium nor high dose "chronic BCAA supplementation was not effective in improving the main parameters indicative of central fatigue" (Falavigna. 2013) - well, at least as long as we still stick to the hypothesis that the latter is induced by the accumulation of 5-HT in the brain.

    Forget about tryptophan and serotonin, focus on ammonia

    The fact that neither the high, nor medium dose of BCAAs did exert any effects on the serotonin levels in the brain does yet not explain why the medium dose supplementation regimen produced ergogenic, while the high dose regimen induced ergolytic effects.

    The occurrence of direct toxic effects due to (too) high amounts of branched-chain amino acids can be ruled out based on previous studies in which the administration of more than 10g/kg body weight of BCAAs (the human equivalent would be 130g+ per day), as well as dosages of 2.5g/kg body weight chronically did not entail any toxic side effects (Shimomura.  2004). The same is true for other confounding variables, such as the citrate synthase activity, a measure of the general efficiency of the training protocol, bood glucose, insulin,free fatty acids, and lactate levels, as well as liver and muscle glycogen content, which were virtually identical in both groups. This leaves us with the increase in plasma ammonia as our 'last resort' to explain the -58% shorter swimming time in the high (S2) vs. medium (S1) dose BCAA group (-43% lower vs. non-supplemented control).

    Figure 2: The reduced performance of the high BCAA group could well be related to peripheral and/or central ammonia build-up as a results of increased BCAA oxidation, camparably low glutamine intakes and the rate-limited enzymantic conversion and recycling of gluatmine (illustration originally from Earrante. 2003). Studies by Snow (2000) and Carvalho-Peixoto (2007) suggest: Both carbohydrate & glutamine supplements could help.
    Based on what we know about the mammalian body, the increased build-up of ammonia in the high BCAA group could be a result of the unfortunate combination of temporary energy shortage and learned wastefulness' in a situation, where the otherwise sparse BCAAs are available in abundance. Furthermore, with a glutamine content of only 9-13% in the casein fraction of their diets (Swails. 1992), the rodents in the high BCAA group did ingest more than 2.6-3.8 times more BCAAs than glutamine; a fact which may have contributed to a temporary glutamine deficiency as a result of its increased use in the detoxification of the ammonia that's generated when the BCAAs are oxidized. The resulting peripheral and possibly central ammonia build-up (see figure 2) could then have begun to intoxicate liver and brains of the rodents and thus hampered gluconeogensis (normal levels stimulate, high levels of ammonia hamper gluconeogensis; cf. Fritz. 1988) and induced central fatigue (Wagenmakers. 1990; Nybo. 2004) -- and that not despite, but rather due to the chronic "high dose" BCAA supplementation (HED ~50g/day).

    So do I have to drop my BCAAs now or what? Whether these results are relevant for you will probably depend on a whole host of parameters, which include
    • the type, intensity and duration of exercise you do, 
    • the ratio of BCAAs to glutamine in your diet,
    • the amount of arginine, which acts as a substrate for the urea cycle and is therefore necessary to for the excretion of ammonia by the kindeys (Schaefer. 2002),
    • the amount of carbohydrates in your diet (with more = less amino acid oxidation = lower ammonia and very low carb = you are in trouble; e.g. Czarnowski. 1995; Snow. 2000; Carvalho-Peixoto. 2007), 
    ... and those factors I will probably have forgotten to mention now. Unless you don't forget that you can neither lifve from BCCAs and protein alone, but accept the neflglected truth that too much protein is about as bad a too little protein, you can file this post under "show your stupid friends" and get back out, when they complain about feeling sick, bloated and fat "despite" eating a BCAA supplemented high protein, low carb (and often even low fat) diets.

    References:
    • Carvalho-Peixoto J, Alves RC, Cameron LC. Glutamine and carbohydrate supplements reduce ammonemia increase during endurance field exercise. Appl Physiol Nutr Metab. 2007 Dec;32(6):1186-90.
    • Errante LD, Petroff OA. Acute effects of gabapentin and pregabalin on rat forebrain cellular GABA, glutamate, and glutamine concentrations. Seizure. 2003 Jul;12(5):300-6.
    • Falavigna G, de Araú jo Junior JA, Rogero MM, de Oliveira Pires IS, rio Graç a Pedrosa R, Martins Junior E, Alves de Castro I, Tirapegui J. Effects of Diets Supplemented with Branched-Chain Amino Acids on the Performance and Fatigue Mechanisms of Rats Submitted to Prolonged Physical Exercise. Nutrients 2013. 4; 1767-1780.
    • Fritz S, Bohnensack R. Stimulation of alanine metabolism in rat liver by ammonia. Biomed Biochim Acta. 1988;47(12):923-32.
    • Meeusen R, Watson P. Amino acids and the brain: do they play a role in "central fatigue"? Int J Sport Nutr Exerc Metab. 2007 Aug;17 Suppl:S37-46.
    • Nybo L, Dalsgaard MK, Steensberg A, Møller K, Secher NH. Cerebral ammonia uptake and accumulation during prolonged exercise in humans. J Physiol. 2005 Feb 15;563(Pt 1):285-90. Epub 2004 Dec 20. 
    • Schaefer A, Piquard F, Geny B, Doutreleau S, Lampert E, Mettauer B, Lonsdorfer J. L-arginine reduces exercise-induced increase in plasma lactate and ammonia. Int J Sports Med. 2002 Aug;23(6):403-7.
    • Shimomura, Y.; Murakami, T.; Nakai, N.; Nagasaki, M.; Harris, R.A. Exercise promotes BCAA catabolism:  Effects  of BCAA supplementation on skeletal muscle during exercise.  J. Nutr.  2004, 134, 1583S–1587S.
    • Snow RJ, Carey MF, Stathis CG, Febbraio MA, Hargreaves M. Effect of carbohydrate ingestion on ammonia metabolism during exercise in humans. J Appl Physiol. 2000 May;88(5):1576-80.
    • Swails WS, Bell SJ, Borlase BC, Forse RA, Blackburn GL. Glutamine content of whole proteins: implications for enteral formulas. Nutr Clin Pract. 1992 Apr;7(2):77-80.
    • Wagenmakers AJ, Coakley JH, Edwards RH. Metabolism of branched-chain amino acids and ammonia during exercise: clues from McArdle's disease. Int J Sports Med. 1990 May;11 Suppl 2:S101-13.

    Friday, November 22, 2013

    Adelfo Cerame: Contest Prep Update on Turkey Day! Plus: Losing Fat W/ Intermittent Fasting & 200g Carbs/Day Works

    When you look at these images yo will have to concede that Adelfo brings a better conditioning to the Thanksgiving table than many a competitor to the stage ;-)
    In a way it's funny how the Internet has brought us all so close together and yet we still have so little in common, when we are not "on the line". I believe it was two weeks ago, when I almost missed my own radio show on Thursday, because I assumed there wouldn't be a SuppVersity Science Round-Up on a holiday. Literally in the last minute I thought, maybe you better check whether the US guys have a holiday, as well - and what should I say? You didn't! Today, I am just back from the office and do now have an 'evening off', 'cause today it's you (or at least ~65% of you, which is the relative amount of US visitors in the last week), who have a holiday that' leaving me somewhat clueless why you are giving thanks and I am not ;-)

    In the end, it does not matter for me what the deeper meaning of Thanksgiving is, as long as I know that you spend the day with your loved ones, it's a day to celebrate and that's all that counts - so HAPPY THANKSGIVING! Enjoy Adelfo's short progress update and get your share of the holiday roast - even if it will have it's share in the 0.5kg the average college student in a 2006 study by Hull et al. gained during the Thanksgiving holiday (. I will make sure there is "Get Lean and Stay Lean Quickie" either on Sunday or Monday, just in case ;-)

    Happy Turkey Day SuppVersity readers! 

    I’m going to make this short and sweet since I know most of you will be celebrating the Thanksgiving holiday with loved ones and eating good food, because I know I will. I’ve been getting some comments and request from a few people for an update on my progress, and I know I have not done one in a while, so this will be just a quick update on my diet, training and progress pictures… I promise that’s all!
    Figure 1: Adelfo's current total caloric intake and macronutrient ratio (left); Adelfo's latest progress pics (right) comparing photos from week 2 and week 7 of this contest prep (img. Adelfo Cerame Jr. 2013).
    Trainingwise, I am still following the Hybrid of P.H.A.T. training using an RPE scale with 3 to 4 workouts per week depending on what day workouts land (click here to learn more about Adelfo's routine). In addition I am doing cardio twice a week: 9 laps around a trace. Everything is pretty much the same since I started in October - the aforementioned cardio is in fact he only addition I have made to my regimen at the beginning of this month.

    One thing I do notice is that my carb intake is a lot higher this year and has not yet to change. By this time last year I was probably already at 125-150g of carbs. I never realized that I was able to maintain 200g of carbohydrates (which is big for me) for a long period of time while still dropping body fat. 

    When I come to think about it, it's probably less that I could not do it, but rather that I never even gave the 200g of carbs per day a chance. Ever since working with coach Alberto, I’ve learned that dieting and listening to your body is like a game of wits, and sometimes, the best move is no move, at all.
    Image 1: Progress picture (backshots) comparing week 2 and week 7 of this competition (Adelfo Cerame. 2013)
    With regards to my training split, it’s still kicking my ass day in day out. I’m exhausted most days every time I stroll out the gym after a training session. I feel sore more than usual nowadays, and my muscles feel tight like I’m somewhat pumped all day long. After mentioning this to Alberto and maybe second guessing myself that it was just in my head; he assures me that it’s not in my head…
    “Just pushing you a bit on the frequency tip which is important for an athlete of your level. We will go back and fourth with it, and you are NOT imagining what you feel dude. Your muscle are under constant turnover now, and because of this I think you will keep a much fuller look.”
    Here is another conversation between coach Alberto and I, when I mentioned still feeling sore and tight but was feeling and noticing more density in my frame especially in my chest area because I feel that the chest area is my weakest body part…
    “Yep! It was about time you rode the frequency train. It’s going to pay off! This week we are going to not change a thing, since we added more cardio. I want to cruise a bit this month December push a bit, but right now it’s coming off easy. We will have some good detail in your back by the end of the month.

    So far so good, and given the new frequency you are working with I think you are going to really see you keep more even though you got leaner”
    But yes… I am very pleased with my training and how everything is coming along. I am confident that I will be able to outdo my old self this coming March of 2013.
    Image 2: Progress picture (front double biceps) week 2 vs. week 7 of this competition (Adelfo Cerame. 2013)
    That’s all I have for this Thursday edition but since it is the holidays, just in case you didn’t get to read it last year. I wrote an article on “5 simple rules to survive the feast without too much damage” So for those of you who just started following my blogs; here is the link to the respective article.

    Have A blessed Holiday SuppVersity readers!


    References:
    • Hull HR, Hester CN, Fields DA. The effect of the holiday season on body weight and composition in college students. Nutr Metab (Lond). 2006 Dec 28;3:44.

    Thursday, November 21, 2013

    "Just One More Set" (2/2): Three Sets of Three Exercises Three Times Per Week - High Volume Can Work. With Appropriate Rest Also to Build Strength & Power

    High , not insane (!) volume training can be productive.
    I hope that you have already being waiting for this post, so I'll try to cut myself short and get right to the facts. In yesterday's first part of "Just One More Rep" it turned out that a higher training volume sucks, when it comes to what is often thought would be its prerogative, i.e. using strength training to induce excess post-exercise oxygen consumption (EPOC) and lean out.

    In view of these results you could argue that it would be totally logical that a higher training volume cannot be ideal for muscle gains either. After all those require energy and if the RMR does not go up, this would suggest that there was little to repair and supercompensate. A recent study (Naclerio. 2013) does yet refute this already intrinsically non-stringent considerations.

    High volume can work! As long as it's high, and not simply insane.

    The study was conducted by researchers from the University of Greenwich, the College of New Jersey, the European University of Madrid and the Appalachian State University and it has one caveat I don't want to hold back to the discussion of the results at the end of this post (although it will reappear and be addressed there): Though we are dealing with college athletes (20 male soccer and 12 female volleyball college players)  none of them had previous strength training experience. This may not be exactly representative of a dumb- & barbell god like you are *rofl*, but is at least better than taking totally untrained participants, where you never know if the the higher volume was too demanding for their musculature or their overall conditioning.

    Moreover, the separate analysis of upper and lower body strength gains could shed some more light on whether or not legs do in fact need some more hammering to adapt than the smaller musculature of the upper body (cf. "Three is more than one").

    Who and at which intensity for how many sets and reps?

    The 32 athletes (age = 23.1± 1.57 yrs, injury free) with at least 3 years of experience as regular team sports practitioner were randomly assigned to one of four groups (all performed 8 reps at 75% of their 1-RM max per set!):
    • low volume(LV), 1 set per exercise and 3 sets per muscle group per session;
    • moderate volume (MV), 2 sets per exercise and 6 sets per muscle group per session;   
    • high volume (HV), 3 sets per exercise and 9 sets per muscle group; 
    and of course, the obligatory non-exercise control group (this leaves us with only 8 subjects per group, so don't expect all too meaningful p-values). Before and after the 6 week each subject underwent a progressive resistance test aimed to determine the 1RM and the maximal average power produced from light to heavy weights on two upper body exercises (bench press (BP) and upright row (UR)) and one lower body exercise (parallel squat (SQ)). The individual tests, were structured as follows:
    "After a standardized warm up, each subject started the PRT  which consisted of 8 sets of 2 repetitions performed with maximal acceleration, alternating with rest periods between 2 min for the light load, 3 to 4 min for the moderate load and 5 minutes for the higher load. The 1st and 2nd sets were performed with a light weight (~25 to 45% of estimated 1RM), the 3rd and 4th sets with a medium weight (~50% to 65% of estimated 1RM), the 5th and 6th sets with a medium to heavy weight (~ 70% to 80% of estimated 1RM), and the 7th and 8th sets with a maximum or near maximum weight (~85% to 100% of estimated 1RM)." (Naclerio. 2013)
    For the subsequent analysis the scientists picked those sets and reps, on which the subjects had lifted with the greatest average power (no sure whether this was the best idea, but alas).

    The workouts - chest, shoulders, biceps + legs, back, triceps

    It stands to reason that the exercises that were part of the testing procedure, i.e. bench presses (BP) and upright rows (UR), which were performed using Olympic bars and plates, as well as the classic back squat (till thighs were parallel to the floor, SQ), which was performed on a Smith machine, "in order to standardize exercise", were also the core exercises of the  actual workouts, the participants performed during the 6-week training phase.
    Day 1 (chest, shoulders, biceps)Day 2 (legs, back, triceps)
    Bench press
    Incline Bench press
    Dumbbell Fly
    Upright Row
    Lateral Raise
    Posterior Lateral Raise
    Barbell Biceps Curl
    Dumbbell Biceps Curl
    Machine Biceps Curl
    Smith Machine Parallel Squat
    Leg Press
    Knee Extension
    Lat Pull down
    Seated Row
    1 Arm Dumbbell Row
    Machine Triceps Extension
    Standing Triceps Pushdown
    1 Arm Triceps Extension
    Table 1: Workout schedule, for set and rep scheme see text above .
    Overall each subject took part in 18 training session, i.e. 3 per week. The training sessions were scheduled on non-consecutive days in a day 1 v.s day 2 fashion, with day 1 being 'chest + shoulder + biceps day ' and day 2 being 'legs + back  + triceps day'. Given the aforementioned volume prescriptions you see that the actual routines were actually more or less representative of what you will see the relatively sane part of the trainees actually do at the gym.
    Figure 1: Relative change (in % of baseline) in 1RM and maximal average power during the 6-week intervention period (Naclerio. 2013)
    If you take into account that these were the first real lifting sessions for most of the study participants, the same can be said of the strength gains I plotted relative to the respective baseline levels in figure 1. In fact, the multivariate analysis the scientists conducted showed that all training protocols yielded statistically significant increases in strength.

    When gains are the goal: Volume (or stimulus?) does count!

    In contrast to the EPOC values, of which we have learned yesterday that they do by no means benefit from increases in total workout volume, Naclerio et al. did actually observe a clear trend toward greater improvement in strength and power with the high vs. the low and even the medium volume protocol (at a similar overall volume, though with different exercises in a classic split routine). Thte most evident downsides to the lower volume programs, were
    • no significant increases in the 1-RM squat in both the low and medium volume group, and
    • no significant increases in the average power during the bench press,
    where both, the medium (MV) and high volume (HV) protocols achieved significant before vs. after differences of 10% and 16%, respectively.

    If you look at the overall pattern in figure 1 once more, there is still no clearcut picture emerging. While it does in fact appear as if the high volume routine appears to be in front in the majority of 1-to-1 comparisons, this is mainly based on an analysis of the improvements in maximum strength. With respect to the average power measurements, on the other hand, the authors are (partly) right to point out that you could argue in favor of both the low and medium volume protocols as being "better strategies for enhancing lower body or upper body average power performance." (Naclerio. 2013)

    So is high volume the way to go - or no?

    For the subjects who participated in this study (and maybe some of you), the last mentioned equivalence, if not superiority of the low(er) volume routines (1-2 sets per exercise) the low and medium volume training do in fact appear to be superior to support their regular sports specific training program. After all, mere strength is not so much of an issue in either soccer or volleyball; and given the fact that at least for soccer the lower limb power is what really counts, a low volume strength training approach would, aside from obviously being highly economic, also yield the most pronounced sport specific performance increases.

    It stands to reason: If neither brute strength nor tons of muscle are your goal and strength training is just an adjunct to your sports-specific training, high volume sucks!
    Whether the surprising superiority of the low volume routine as a 'average strength builder'  for soccer and volleyball players does mean that legs need less, rather than more work than the chest, which appears to like the constant hammering, is however highly questionable. In fact, this is where the bias of previous training comes into play. For both volleyball and soccer players, the latter does obviously include a hell lot of 'leg work' and while you do push-ups in soccer (and I guess volleyball as well), there is no training component that would correspond to the sprinting and HIIT exercises that involve the legs only. Now, of the latter you know that they can in fact have 'anabolic' effects on skeletal muscle. These may not be so immediate as they would be for someone doing a BB-like hypertrophy training, but they accumulate over time; and with three years of more or less 'professional' training in their respective sports, we may savely assume that all participants had their share of muscular hypertrophy in the quads, glutes and hams.

    Moroever, skeletal muscle hypertrophy and strength gains require a certain degree of overload. Allegedly, when the training induced or the overall stress becomes too much, your training won't yield the desired results either. For someone whose main goals are skeletal muscle hypertrophy and strength gains, and who does not compete in any other sports that requires separate training,  the data from the study at hand would yet still support Arnold's way of doing "just one more set " - as long, as this is done in conjunction with adequate rest not just in-between sets, but in-between workouts, as well!

    I can however guarantee you that doing 27 sets today and another 27 tomorrow, just to follow that up by some HIIT on day 3, in order to have a "day off" without a guilty conscience and to be "recovered" to do chest shoulders and biceps, your day 1 on day 4 again (thus starting another "cycle"), will yield neither muscle, nor strength gains. It will simply burn you out and pave your way right into the Athletes' Triad.

    References:
    • Naclerio F, Faigenbaum AD, Larumbe-Zabala E, Perez-Bibao T, Kang J, Ratamess NA, Triplett NT. Effects of different resistance training volumes on strength and power in team sport athletes: a pilot study. J Strength Cond Res. 2013 Oct 5.

    Wednesday, November 20, 2013

    "Just One More Set" (1/2): Metabolic Response to 10,000kg vs. 20,000kg Regimen. EPOC: Do Reps and Loads Both Figure? And What About Elite Athletes Do They Need More?

    "Ah come on, just another set!" ... "I don't know man, we've already pumped away 100,000kg today... do you really believe that's going to be productive, I mean, yeah, we are cuttin', but still... I mean I don't dig this epic!", "EPOC man, it's called EPOC!" *shakes his head* "Call it whatever you want, bro, I am out!"
    If you want, you can think of today's SuppVersity post as an extension to yesterday's "Bigger, Stronger, Faster" special of the On Short Notice series; to be more precise: As a practically more relevant version of the rodent study on hypertrophy vs. strength training that was part of the aforementioned post. Yep, we are "talking volume" today. How much is too much?  And though this is never-ending debate, it appears that at least as far as research goes, a little more debating certainly would not hurt. Therefore I am happy to have not one, but two studies for you which don't just address this issue, but have also been conducted with human subjects!

    In view of the fact that these are no "short notices", I will discuss one today and the other tomorrow - yep, that means that you can already make a mental note to come back tomorrow ;-)

    "Just one more set, ..." - how productive can that be?

    Today's study comes from the Human Performance Laboratory at the Florida State University and deals with the energetic side of things - specifically the often-cited EPOC (excess post-exercise oxygen consumption), which is often touted as one of the most important aspects why strength training in general and higher volume / intensity strength training, in particularly, would have the edge over cardio training. The reasoning is easy: You don't burn so much energy while you work out, but in the time after, your body will (a) still expend more energy per minute / hour and (b) has the advantage of emptied glycogen stores, which will force it to tap into its body fat stores the source for the required energy.

    All of you who have read the complete Athletes' Triad series, will by now already know that at least argument (b) is pretty idiotic, because it wouldn't allow you to replenish your muscle and liver glycogen after workouts and thus pave the way into the dreaded vicious circle of the athlete's triad. The former argument, on the other hand has - on a way more general level - only been confirmed a couple of days ago (see "Scientists resolve the paradox of stable muscle metabolism but greater mitochondrial respiration in muscle of inactive vs. active subjects", read more), the question still remains: How much weight do you have to lift to set the 'afterburner' into full gear? 

    10 metric tonne or 20 metric tonnes? What do you say?

    I see, you are laughing, but basically the above question is what the George J. Abboud and his colleagues tried to find out, when they recruited 8 healthy men aged19-29 yrs who had 
    "at least 12 months of RT experience with no more than 2 wks rest at a time, less than a total of 4 wks off within the last 6 months, or 9 wks off within the last 12 months [and] reported no prior or current use of illegal performance enhancing substances." (Abboud. 2013)
    Suggested read "Three is More Than One: Higher Volume Increases Strength Gains in Legs, and Satellite Cell Recruitment and Fiber Size in Legs & Traps."
    As usual the subjects had to fill food logs for the three days before the testing and were instructed to replicate the same eating pattern on the second occasion in which they were randomly assigned to perform a standardized resistance training (RT) regimen consisting of 4 exercises performed on a non-counterbalanced smith machine so that the range of motion during

    • bench press, 
    • squat, 
    • bent-over row and 
    • Romanian deadlift 
    could be controlled for easily. Other than the equipment and the exercises, which were identical on both occasions, the volume of the training sessions varied and if you express this volme in kg or metric tons, it was a competition of 10,000 kg (10 metric tonnes) vs. 20,000 kg (20 metric tonnes) of weight. 
    "The loads were divided between the 4 exercises as follows: 35% to squats, 30% to bench press, 20% to bent-over rows and 15% to Romanian deadlift. For each set, subjects lifted approximately 85% of their 1RM for 6-8 repetitions. If 6 repetitions could not be completed at any point, the load was reduced by 10% for the subsequent set." (Abboud. 2013)
    Both sessions were supervised by three testers : One monitored the metabolic cart, one made sure the proper range of motion was used and one monitored the proper lifting form. The subjects had to perform the concentric portion of each lift with maximal speed and ensure a controlled eccentric descent. A specific time interval was not dictated. Sets were stopped if "subjects broke form" (Abboud. 2013) and 2 minutes of rest were given between sets. In this fashion the subjects simply kept lifting set after set until the volume prescription for the respective trial was reached.
    Figure 1: Resting metabolic rate (RMR) per kg body weight, 30min energy expenditure and respiratory exchange ratio (RER; lower values = higher fat, lower glucose  oxidation)  after low and high volume trial (based on Abboud. 2013)
    As you can see in figure 1 there were differences as far as the effects of the high (20,000) vs. low (10,000kg) regimen on the resting metabolic rate, 30 min energy expenditure, and the respiratory quotient (lower values = higher fat, lower glucose oxidation), but in view of the fact that the high volume group moved 2x more weight and should thus (at least theoretically) have expended twice the energy (assuming they performed all reps with perfect form and identical speed), those differences are more than disappointing. 

    The minuscle effect size is yet not the most "disappointing" (or "surprising" ?) result

    In fact, contrary to the low volume workout the 20,000kg workout did not produce any increases in resting metabolic rate and 30min energy expenditure, at all - put simply: There was no EPOC after the high volume trainingAnd this did not change over the whole 48h period (and I know you guys, you won't rest longer anyway ;-).

    Now you may say that this was a crazy protocol, but let's do the math, let's assume the guys did squat 100kg, benched and rowed their own body weight of ~80kg and deadlifted 125kg. With 10 reps per set thats 1,000kg + 2x800kg + 1,250kg per set respectively. If they did three sets per exercise they would thus already be up to 9,950kg! If you still think that's crazy, let's hear what the scientists have to say:
    "As subjects in the present study were well adapted to RT, the training stimulus needed to elicit increases in EPOC arguably needed to be much higher compared to that used in previous research  Two studies using intensities of 70% 1RM report significant increases in RMR. Melby et al. had subjects perform 6 setsof 10 different exercises for a total of 60 sets. The repetition range for this protocol was 8-12 repetitions per set. This amounts to approximately 600 repetitions performed during the course of the exercise bout. The range of load-volume lifted by these subjects was 15,000-38,000 kg. [...]" (Abboud. 2013)
    The list goes on and you just have to go to your gym and I guarantee you, no matter how few people are on the floor you will see a guy who (often without noticing is) will be pounding away much more word within a single workout. Moreover, the the subjects in the present study completed their trials
    with a drastically lower number of repetitions -  a mean of 199. Had they performed the crazy rep-volume of the Melby study, they would probably have come close to 50,000 kg. This raises an interesting question is "volume" correctly defined by giving the total amount of weight you lift? Or is the number of reps maybe more important as far as the after-burner EPOC is concerned?

    Too much of a good thing? But what if you are a highly trained athlete?

    A previous study, by Hackney et al. would support the notion that heavy lifting is an obligatory part of the EPOC equation. In the latter study, EPOC trained individuals who used a lower load-volume than the trainees in the study at hand  had increased resting metabolic rates for up to 72h (Hackney. 2008). Since the Hackney study also put an emphasis on eccentric contractions and will thus probably have lead to even greater muscle damage than the protocol of the study at hand (CK(10,000kg) = 729U/L vs.CK(20,000kg)  = 1,159IU/L), Abboud et al. speculate that ...
    "[a]s protein synthesis required for repair is energetically expensive, it is logical that untrained subjects will show greater and longer alterations in EPOC post-RT. Judging by training history, strength levels and CK responses, subjects in the present study had most likely reached a higher level of adaptation than ones in previous studies, and therefore were less sensitive to the metabolic effects of recovery from RT." (Abboud. 2013)
    In other words, for you, probably a seasoned strength trainee, the 'more is more' principle is not going to yield better results - even if your goal is to shed body fat. And ...
    "Although RT is an important component in any weight loss program to attenuate the loss of fat free mass and therefore better preserve RMR, it is unlikely that the total energetic cost (during and post-exercise) of a typical duration workout will be adequate for significant weight reduction in highly trained recreational lifters without caloric restriction and/or additional aerobic or high intensity interval training." (Abboud. 2013)
    And since I rarely encounter a conclusion that's so to the point, I'll leave you with that for today and remind you to come back tomorrow to learn, when and for which body parts doing somewhat more may still be beneficial - read me tomorrow ;-)

    References:
    • Abboud GJ, Greer BK, Campbell SC, Panton LB. Effects of Load-Volume on EPOC after Acute Bouts of Resistance Training in Resistance Trained Males. J Strength Cond Res. 2013 Oct 18.
    • Hackney KJ, Engels HJ, and Gretebeck RJ. Resting energy expenditure and delayed-onset muscle soreness after full-body resistance trainingwith an eccentric concentration. J Strength Cond Res. 2008; 22: 1602-1609.
    • Melby C, Scholl C, Edwards G, and Bullough R. Effect of acute resistance exercise on postexercise energy expenditure and resting metabolic rate. J Appl Physiol. 1993; 75: 1847-1853.

    Tuesday, November 19, 2013

    Bigger, Stronger, Faster: CoQ10 for Brain & Muscle in Young & Old. The Optimal HIIT Regimen for Fun & Fat Loss - 8s at 100% 60s Idling! Protein Power From Oats? Plus: Rest Times, Clusters, Form & Hypertrophy Training

    I decided against calling this the "Get Big, Green and Look Like the Hulk Quickie" (img. Paramount Pictures)
    In view of the fact that most of you apparently enjoyed the "Get Lean and Stay Lean Quickies" I posted in the last weeks, I thought you may also be inclined to read a "Get Big, Green and Look Like the Hulk Quickie", but then decided that the name was too long for the headline and "big, green" and maybe even "hulk" in conjunction with "quickie" may have had the SuppVersity turn up on google and other search engines in too close vicinity to websites I do not exactly want this blog to be associated with (just kiddin' ;-)

    Be that as it may, enjoy the ride and let me know whether you do prefer this thematically structured approach over the classic news-potpouris ala On Short Notice.

    I mean you can obviously argue in favor of both and since this is a place I want you to look forward to visit everyday, I would be inclined to hear whether "innovations" like this make it better or worse.
    • CoQ10 supplementations could offer beneficial effects on brain and muscle in elderly individuals and could - at higher doses - work for youngsters, as well (Shetty. 2013) -- In a rodent trial the provision of a relatively high dose of CoQ10 as part of the diet effectively blunted age-induced 'cognitive decline' (whatever that may be in a mouse ;-) and protein breakdown in heart, liver and muscle tissue of the 17.5 months-old mice.

      Figure 1: Carbonyl levels in liver and muscle of young and old mice.
      This result is also interesting, because the mitochondrial protein breakdown in the brain was the one which was least beneficially affected. What's of greater importance, for this news quickie at least, is that the 2.81mg CoQ10 per gram of chow the high dose group received (the human equivalent would be roughly 33mg/kg or 2-3g per day!) elicited beneficial effects on the skeletal muscle carbonyl content, a marker of protein oxidation, in the young animals, as well (see figure 1).

      The notion that coQ10 in appropriate doses could be beneficial not just for statin users, but also for perfectly healthy people, even athletes, is also supported by the beneficial effects the administration of coQ10 had in a recent trial where it blunted the oxidative damage due to a high frequency endurance training program in rodents (Okudan. 2013). It does however stand in contrasts with a recent study by Bloomer et al., n which the supplementation of only 300mg (ca. 15% of the HED used in the study at hand) did not yield the desired effects on the exercise performance of 15 perviously individuals (10 men and 5 women; 30-65 years; Bloomer. 2013). Whether this really is just a matter of the correct dosing, or maybe a fundamental difference between mice and men will still have to be elucidated and I would not spent the bucks for anything that's only "maybe" going to work.
    • Rats don't do well with hypertrophy training (Scheffer. 2013) -- According to a recently published paper by Scheffer et al., rodents who are afraid of increases in markers of oxidation should refrain from hypertrophy oriented muscle training and stick to lower volume resistance training.

      For their study, the Brazilian researchers had analyzed the effects of three different resistance training protocols, namely  muscular resistance training (RT), hypertrophy training (HT), and strength training (ST), which had to be performed twice a week for 12 weeks on muscle lactate and glycogen content, superoxide production, antioxidant enzyme content, and activities, as well as markers of lipid and protein oxidation.
      While you certainly don't want a fire inferno, doing too little is not going to yield the results you are looking for either (suggested read "The emerging role of an auto-/endocrine-immune axes")
      "Results showed increased superoxide production (UT = 5.348; RT = 5.117; HT = 8.412 ; ST = 6.354), SOD (UT = 0.078 ; RT = 0.101 ± 0.013; HT = 0.533 ± 0.109; ST = 0.388), GPx (UT = 0.290; RT = 0.348; HT = 0.529; ST = 0.384) activities, and content of GPx (HT = 3.8 times; ST = 3.0 times) compared with the UT group. CAT activity was lower (UT = 3.966; RT = 3.47; HT = 2.276 ; ST = 2.028) in HT and ST groups. Oxidative damage was observed in the HT group (TBARS = 0.082; carbonyl = 0.73; thiol = 12.78) compared with the UT group.
      The way in which the authors' conclusion that these "findings indicate that HT causes an imbalance in oxidative parameters in favor of pro-oxidants, causing oxidative stress in skeletal muscle" does implicitly suggest that this is a bad thing that has to be avoided at all cost, which is obviously totally beside the point - if you don't challenge your body he has nothing to adapt to and neither your conditioning, nor your strength or muscle mass are going to improve. The absence of statistically significant increases in oxidative damage in the low volume strength training routines, on the other hand supports the notion that you can use phases of very low volume training with heavy weights in between intense phases of overreaching to monetize on the muscular gains (which will probably keep coming in these 2-4 weeks) by taking all your lifts to another level and thus lying the foundation for future growth.
    • Optimized HIIT regimen for overweight kids: 4s max sprinting + 60s active recovery do the trick - could work four you, as well! (Crisp. 2013) -- Either from the SuppVersity news or from listening to Super Human Radio you may remember that a recent study by Deighton et al. has found that sprinting will increase your appetite more and burn less calories than workload matched steady state cardio training. A novel study from Australia does now suggest that this may well be the case, but probably only as a result of doing it wrong - the sprinting, I mean.

      In their study Nicole A. Crisp and her colleagues from the School of Sport Science at the The University of Western Australia, describe how the combination of 8s all-out sprints on a cycle-ergometer with 60s of active rest easily outperforms its 30min steady state counterpart (30min moderate pace) and its "little brother" and "big" bother the 8s sprint, 120s active rest and the 8s sprint, 30s active rest protocol, respectively.

      This is not the first study to show the superiority of HIIT training in obese boys. In February 2013, I have already covered a very similar study where a brief HIIT session that burns "only" 360kcal was a major motor to teenage weight loos.
      In that, it's important to note that despite a linear increase in energy expenditure from the 60s to the 30s rest group, the latter failed to reach statistical significance (p = 0.076), ...
      "[...] likely as a result of decreased sprint quality as indicated by a significant decline in peak power output from SI60 to SI30 (p = 0.034)" (Crisp. 2013)
      In conjunction with the absence of the overcompensation effect at the following breakfast buffet (which was present in the 8/30s and to a lesser degree even the 30min steady state trial), this makes the 8/60s protocol the superior choice for everyone doing HIIT mainly to get or stay lean.

      And aside from its practical value, the 8/60s regimen was also the one the boys enjoyed the most.

      "And what about me?" I don't see why doing this very short sprints with large bouts of active recovery in between would not be right for you, as well. At least if the activation of your metabolism and / or fat loss during a diet is your primary goal and not an improvement in VO2 max., you would thus probably get many of the benefits at a smaller risk of overtraining. Just make sure you are actually sprinting all-out for the whole 8s and don't stop, when you are just about to get up to speed ;-)
    • Long rest times necessary for high jumpers - with 8-12 min pause your performance on the next jump will increase not decrease (Gouvêa . 2013) -- In a recent meta-analysis that's soon going to be published in the Journal of Sports Sciences André Luiz Gouvêa and his co-workers report that the manipulation of rest intervals seems to affect post-activation potentiation magnitude and jump height.

      After analyzing fourteen studies, which met the criteria of having a crossover design, being randomised, or non-randomised and counterbalanced and observed the voluntary muscle action-induced post-activation potentiation on jumping performance, the scientists state that the...
      "[...]results demonstrated medium effect sizes for rest intervals 0–3 and 8–12 min (-0.25 for 0–3 min; 0.24 for 8–12 min) and a small effect for other ranges (0.15, CI: -0.08 to 0.38 for 4–7 min; for ≥16 min)." (Gouvêa . 2013)
      Since there was little to no evidence for heterogeneity among the sub-groups and no indication of publication bias, these results clearly suggest that rest intervals of 0–3 min have detrimental, rest intervals between 4-8 min neutral and rest intervals of 8-12 min beneficial impact on jump height. In that, the improvements are likely to be mediated by post-activation potentiation.
    • The science of cluster training - Conventional wisdom prevails, 10 deep breaths would yield optimal rest time between reps (Hardee. 2013) -- At lest with respect to proper form, which may also have consequences for optimal muscle recruitment and hypertrophy, the optimal rest time between two reps in a cluster appears to be 20s, which is actually what the good old "10 deep breaths rule" would state, as well.

      What is a cluster? When you are "clustering" your sets you are doing one (sometimes also 2-3) rep(s), rack the weight, rest, do another rep etc. This is a very good technique to increase your strength and break through plateaus that's useful for powerlifters and bodybuilders alike.
      In order to examine the effects of three different cluster set configurations on power clean technique, the scientists recruited 10 male, recreational weightlifters who had to perform clustered or non-clustered power-cleasn: 3 sets of 6 repetitions at 80% of their individual repetition max with 0 (P0), 20 (P20) or 40 seconds (P40) inter-repetition rest.

      In the P0 (no cluster) condition, the scientists observed how the form suffered and got worse and worse from the first to the last rep (the catch and first pull were in a more forward position during repetition 6 as compared to repetition 1). With the long rest times in the P40 condition, on the other hand,  "differences in horizontal displacement were found between repetitions 1 and 6 for the second pull and the loop" (Hardee. 2013).

      In the P20 condition the researchers did not observe any differences in horizontal displacement between repetitions 1 and 6 during P20. Reason enough for the scientists to conclude that their results would demonstrate that cluster sets with greater tha 20s of inter-repetition rest would be useful to maintain appropriate form without significantly dropping the density of your workout - whether this did actually facilitate strength or size gains was yet not evaluated in the study.

      The evidence for the efficacy of this training regimen (aside from simply providing a novel stimulus, which is obviously always a good thing) does come from a 2011 study by  Hansen et al who compared the response of highly trained rugby players to on traditional vs. clustered leg training program and found the latter to be slightly superior with respect to the increase in peak velocity and strength , while the classic continouus set training had an edge as far as total power increases in the lower limbs were concerned (Hansen. 2011).
    • Oats healthy car source with performance protein? (Xu. 2013) Who would have thought that, oats are not just an excellent source of slow digesting carbs and potentially fat-burning beta glucan (listen to the SuppVersity Science Round-Up from two weeks ago), they also contain a non-negligible amount of protein of which researchers from the College of Food Science and Engineering at the Northwest A&F University in Yangling, China, have now shown that it ameliorates the increase in lipid oxidation (MDA) and decrease in antioxidant activity (SOD) during an exaustive bout of swimming in 30 male mice.

      After the oat protein had been purified from oat meal thirty male Kun-ming mice were divided kept on either a normal control diet, a diet that was enriched with oatmeal and a diet that contained only the protein fraction of the oats. After 20 days, the rodents were subjected to swim to exhaustion. Their swimming endurance and the major metabolic substrates were measured from serum, liver and muscle.
      Figure 2: Antioxidant enzymes up, lipid oxidation down. That's the result of a comparison of the detrimental effects of an exhaustive swimming test in mice fed regular, oat enriched or oat protein pimped diets for 30 days (Xu. 2013). What the study cannot answer is however whether identical effects would have been seen w/ any other high quality protein as.
      "The results showed that no significant differences were observed in swimming endurance test between the normal control group and the oat protein group (P > 0.05). Mice in the oat meal group had significantly longer swimming endurance compared to the normal control group (P < 0.05). Furthermore, dietary oat protein increased the levels of liver glycogen, enhanced the activities of lactic dehydrogenase and superoxide dismutase, and decreased the levels of blood urea nitrogen and malondialdehyde in serum." (Xu. 2013)
      Now the good news certainly is that id did work. The "bad" or at least not so good news, on the other hand is that there is no adequate control group, since the addition of both the oats and the oat protein will have increased the amount of essential amino acids in the rodent chow significantly.
    That's it! At least as far as news on getting bigger, stronger and faster is concerned. In case yo are also interested in something else, for example...
    and whatever else I will still be posting before tomorrow's SuppVersity news, you are alway welcome to visit the SuppVersity Facebook Wall.

      References:
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      • Scheffer DL, Silva LA, Tromm CB, da Rosa GL, Silveira PCL, de Souza CT, Latini A, Pinho RA. Impact of different resistance training protocols on muscular oxidative stress parameters. Applied Physiology, Nutrition, and Metabolism, 10.1139/h2013-115
      • Shetty RA, Forster MJ, Sumien N. Coenzyme Q(10) supplementation reverses age-related impairments in spatial learning and lowers protein oxidation. Age (Dordr). 2013 Nov 10. 
      • Xu C, Lv J, You S, Zhao Q, Chen X, Hu X. Supplementation with oat protein ameliorates exercise-induced fatigue in mice. Food Funct. 2013 Nov 12.